Quantitative analysis of double-strand break in giant DNA by single-molecule observation

被引：0

作者：

Yoshikawa, Y. ^{[1
]}

Mori, T. ^{[2
]}

Yoshikawa, K. ^{[3
]}

机构：

[1] Nagoya Bunri Coll, Dept Food & Nutr, Nagoya, Aichi 4510077, Japan

[2] Osaka Prefecture Univ, Radiat Res Lab, Sakai, Osaka 5998570, Japan

[3] Kyoto Univ, Dept Phys, Kyoto 6068502, Japan

来源：

PROCEEDINGS OF THE XIII CONGRESS OF THE SOCIETY FOR FREE RADICAL RESEARCH INTERNATIONAL | 2006年

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have performed a real-time observation of photo-induced double-strand breaks of giant DNAs stained by a cyanine dye, YOYO, under intense illumination in solution using fluorescence microscopy. YOYO is known to have the ability to photo-cleave DNAs by generating reactive oxygen species such as hydroxyl radicals. We have also investigated the effect of antioxidants such as a water-solubule flavonoid, glucosyl-hesperidin, and ascorbic acid (vitamin Q on preventing double-strand breaks in a quantitative manner. It is stressed that the experimental methodology of single chain observation serves as a useful tool for studying the double-strand break of giant DNA.

引用

页码：341 / +

页数：3

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