Quantitative analysis of double-strand break in giant DNA by single-molecule observation

被引：0
|
作者：
Yoshikawa, Y. [1 ]
Mori, T. [2 ]
Yoshikawa, K. [3 ]
机构：
[1] Nagoya Bunri Coll, Dept Food & Nutr, Nagoya, Aichi 4510077, Japan
[2] Osaka Prefecture Univ, Radiat Res Lab, Sakai, Osaka 5998570, Japan
[3] Kyoto Univ, Dept Phys, Kyoto 6068502, Japan
来源：
PROCEEDINGS OF THE XIII CONGRESS OF THE SOCIETY FOR FREE RADICAL RESEARCH INTERNATIONAL | 2006年
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中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have performed a real-time observation of photo-induced double-strand breaks of giant DNAs stained by a cyanine dye, YOYO, under intense illumination in solution using fluorescence microscopy. YOYO is known to have the ability to photo-cleave DNAs by generating reactive oxygen species such as hydroxyl radicals. We have also investigated the effect of antioxidants such as a water-solubule flavonoid, glucosyl-hesperidin, and ascorbic acid (vitamin Q on preventing double-strand breaks in a quantitative manner. It is stressed that the experimental methodology of single chain observation serves as a useful tool for studying the double-strand break of giant DNA.
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页码：341 / +
页数：3
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