Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene

被引：4
|
作者：
Marote, Ana [1 ,2 ]
Pomeshchik, Yuriy [3 ,4 ,5 ]
Collin, Anna [6 ]
Goldwurm, Stefano [7 ]
Lamas, Nuno J. [1 ,2 ,9 ]
Pinto, Luisa [1 ,2 ,8 ]
Salgado, Antonio J. [1 ,2 ]
Roybon, Laurent [3 ,4 ,5 ]
机构：
[1] Univ Minho, Sch Med, Life & Hlth Sci Res Inst ICVS, Braga, Portugal
[2] PT Govt Associate Lab, ICVS 3Bs, Braga, Guimaraes, Portugal
[3] Lund Univ, Dept Expt Med Sci, Wallenberg Neurosci Ctr, Stem Cell Lab CNS Dis Modeling, BMC A10, Lund, Sweden
[4] Lund Univ, Strateg Res Area MultiPk, Lund, Sweden
[5] Lund Univ, Lund Stem Cell Ctr, Lund, Sweden
[6] Off Med Serv, Dept Clin Genet & Pathol, Div Lab Med, Lund, Sweden
[7] ASST PINI CTO, Parkinson Inst, Milan, Italy
[8] Behav & Mol Lab, BnML, Braga, Portugal
[9] Braga Hosp, Dept Anat Pathol, Braga, Portugal
来源：
STEM CELL RESEARCH | 2018年 / 28卷
基金：
瑞典研究理事会;
关键词：
D O I：
10.1016/j.scr.2018.01.022
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD. (c) 2017 Published by Elsevier B.V.
引用
收藏
页码：44 / 47
页数：4
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