Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes

被引：1

作者：

Fu, Wandong ^{[1
,2
]}

Yao, Jianting ^{[1
,2
]}

Wang, Xiuliang ^{[1
]}

Liu, Fuli ^{[1
,2
]}

Chong, Zhuo ^{[1
,2
]}

Duan, Delin ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2008年 / 26卷 / 02期

关键词：

Brown alga; Laminaria japonica; Plastid DNA; Ultracentrifugation;

D O I：

10.1007/s11105-008-0028-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.

引用

页码：114 / 120

页数：7

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