Establishment of an agroinoculation system for broad bean wilt virus 2

被引：15
|
作者：
Atsumi, Go [1 ]
Tomita, Reiko [1 ]
Kobayashi, Kappei [2 ]
Sekine, Ken-Taro [1 ]
机构：
[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan
[2] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan
来源：
ARCHIVES OF VIROLOGY | 2013年 / 158卷 / 07期
关键词：
NUCLEOTIDE-SEQUENCE; CHINESE ISOLATE; CDNA; AGROINFECTION; VERSATILE; PLANTS; RNA;
D O I：
10.1007/s00705-013-1625-7
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.
引用
收藏
页码：1549 / 1554
页数：6
相关论文
共 50 条
  • [21] Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants
    Vicente Medina
    Caterina Carpino
    Laura Elvira-González
    Luis Rubio
    L Galipienso
    European Journal of Plant Pathology, 2022, 164 : 451 - 459
  • [22] First report of Broad bean wilt virus 1 in Slovenia
    Mehle, N.
    Tusek Znidaric, M.
    Tornos, T.
    Ravnikar, M.
    PLANT PATHOLOGY, 2008, 57 (02) : 395 - 395
  • [23] Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants
    Medina, Vicente
    Carpino, Caterina
    Elvira-Gonzalez, Laura
    Rubio, Luis
    Galipienso, L.
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2022, 164 (03) : 451 - 459
  • [24] SPOTTED WILT OF BROAD BEAN
    YU, TF
    PHYTOPATHOLOGY, 1947, 37 (03) : 191 - 192
  • [25] FIRST REPORT OF NATURAL INFECTION OF BROAD BEAN WILT VIRUS 2 ON LACTUCA INDICA
    Wu, X. Y.
    Wu, J. X.
    Cheng, X. F.
    JOURNAL OF PLANT PATHOLOGY, 2010, 92 (04) : S119 - S119
  • [26] First Report of Broad Bean Wilt Virus 2 in Marigold (Tagetes erecta) in China
    Dong, Zheng
    Abuduwaili, Annihaer
    Wang, Xulong
    Yin, Hang
    Lu, Shuhao
    Xia, Fei
    Bi, Yang
    Li, Yongqiang
    PLANT DISEASE, 2021, 105 (11) : 3767 - 3767
  • [27] Hybrid RNA sequencing of broad bean wilt virus 2 from faba beans
    Maina, Solomon
    Norton, Sally L.
    Rodoni, Brendan C.
    MICROBIOLOGY SPECTRUM, 2023, 11 (06):
  • [28] Development of a Broad bean wilt virus 2-based expression vector for gentian
    Tasaki, Keisuke
    Atsumi, Go
    Nishihara, Masahiro
    Sekine, Ken-Taro
    SCIENTIA HORTICULTURAE, 2016, 201 : 279 - 286
  • [29] Genetic Compositions of Broad bean wilt virus 2 Infecting Red Pepper in Korea
    Kwak, Hae-Ryun
    Kim, Mi-Kyeong
    Nam, Moon
    Kim, Jeong-Soo
    Kim, Kook-Hyung
    Cha, Byeongjin
    Choi, Hong-Soo
    PLANT PATHOLOGY JOURNAL, 2013, 29 (03): : 274 - 284
  • [30] First report of Broad bean wilt virus 2 in the UK:: findings in foxglove and salvia
    Mumford, R. A.
    Jarvis, B.
    Harju, V.
    Boonham, N.
    Skelton, A.
    PLANT PATHOLOGY, 2006, 55 (06) : 819 - 819
12345