Establishment of an agroinoculation system for broad bean wilt virus 2

被引：15
|
作者：
Atsumi, Go [1 ]
Tomita, Reiko [1 ]
Kobayashi, Kappei [2 ]
Sekine, Ken-Taro [1 ]
机构：
[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan
[2] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan
来源：
ARCHIVES OF VIROLOGY | 2013年 / 158卷 / 07期
关键词：
NUCLEOTIDE-SEQUENCE; CHINESE ISOLATE; CDNA; AGROINFECTION; VERSATILE; PLANTS; RNA;
D O I：
10.1007/s00705-013-1625-7
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.
引用
收藏
页码：1549 / 1554
页数：6
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