In vitro and in vivo anti-influenza A virus activity of antisense oligonucleotides

被引：7
|
作者：
Abe, T [1 ]
Mizuta, T
Suzuki, S
Hatta, T
Takai, K
Yokota, T
Takaku, H
机构：
[1] Chiba Inst Technol, Dept Ind Chem, Chiba 2750016, Japan
[2] Rat Drug Design Labs, Fukushima 9601242, Japan
来源：
NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 6-7期
关键词：
D O I：
10.1080/07328319908044823
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have demonstrated that antisense phosphorothioate oligonucleotides (S-ODNs) inhibit influenza virus A replication in MDCK cells. The liposomally encapsulated and the free antisense phosphorothioate oligonucleotides with four target sites (PB1, PB2, PA, and NP) were tested for their abilities to inhibit virus-induced cytopathogenic effects by a MTT assay using MDCK cells. The liposomally encapsulated S-ODN complementary to the sites of the PB2-AUG initiation codon showed highly inhibitory effects. Therefore, the antiviral effects of S-ODN-PB2-AUG and PA-AUG were examined in a mouse model of influenza virus A infection. PB2-AUG oligomer treated i.v. significantly prolonged the mean survival time in day (MDS) and increased the survival rates with does dependent manner.
引用
收藏
页码：1685 / 1688
页数：4
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