In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC50 values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 mu g/kg (CLIN) and 6.8 mu g/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra -/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC - MS/MS with a correlation coefficient >0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles. (C) 2016 Elsevier Ltd. All rights reserved.
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NW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China
Zhang, Jing
Feng, Gang
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NW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China
Feng, Gang
Luo, Li
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NW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China
Luo, Li
Yu, Xiang Yang
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Jiangsu Acad Agr Sci, Food Safety & Serv Ctr, Nanjing 210014, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China
Yu, Xiang Yang
Ma, Zhi Qing
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NW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China
Ma, Zhi Qing
Feng, Jun Tao
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NW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China
Feng, Jun Tao
Liu, Xian Jin
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Jiangsu Acad Agr Sci, Food Safety & Serv Ctr, Nanjing 210014, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China
Liu, Xian Jin
Zhang, Xing
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NW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R ChinaNW A & F Univ, R&D Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China