The protective effect of α-tocopherol and GdCl3 against hepatic ischemia/reperfusion injury

被引:44
|
作者
Giakoustidis, D
Papageorgiou, G
Iliadis, S
Giakoustidis, A
Kostopoulou, E
Kontos, N
Botsoglou, E
Tsantilas, D
Papanikolaou, V
Takoudas, D
机构
[1] Aristotle Univ Thessaloniki, Hippokrat Hosp, Dept Transplantat Surg, Thessaloniki, Greece
[2] Aristotle Univ Thessaloniki, Hippokrat Hosp, Dept Biol Chem, Thessaloniki, Greece
[3] Aristotle Univ Thessaloniki, Hippokrat Hosp, Sch Med, Dept Pathol & Histol, Thessaloniki, Greece
关键词
alpha-tocopherol; gadolinium chloride; liver ischemia/reperfusion; lipid peroxidation;
D O I
10.1007/s00595-005-3162-9
中图分类号
R61 [外科手术学];
学科分类号
摘要
Purpose: To evaluate the combined effect of alpha-tocopherol and gadolinium chloride (GdCl3) in reducing lipid peroxidation after severe hepatic ischemia/reperfusion (IR) injury. Methods: Sixty male Wistar rats, 200-250eg, were randomly divided into six equal groups. There were two sham operation (SHAM) groups, two untreated IR groups, and two IR groups treated with GdCl3 and alpha-tocopherol (IRGT). After 60 min of total hepatic ischemia and 120 min reperfusion, one of each group was killed, liver samples were taken for malondialdehyde (MDA) and myeloperoxidase (MPO) analysis and light microscopy examination, and blood samples were analyzed for aspartate (AST) and alanine (ALT) transaminase, lactate dehydrogenase (LDH), and alpha-tocopherol content. The remaining groups were monitored for survival rate determination. Results: The mean MDA and MPO values in the SHAM, IR, and IRGT groups, respectively, were 1.117, 1.476, and 0.978 nmol/g wet tissue and 1.49, 6.26, and 1.78 (U/g). The mean alpha-tocopherol values in the SHAM, IR, and IRGT groups, respectively, were 10.4, 1.9, and 12 mu mol/l. The mean serum AST, ALT, and LDH values were significantly higher in the IR group than in the SHAM group (P < 0.001), and significantly lower in the IRGT group than in the IR group (P < 0.001). Light microscopy examination revealed more severe congestion and vacuolization in the IR group than in the SHAM group, and minimal congestion and vacuolization in the IRGT group. Survival was significantly higher in the IRGT group than in the IR group. Conclusions: The administration of GdCl3 and alpha-tocopherol is likely to protect the liver against lipid peroxidation by suppressing Kupffer cell and polymorphonuclear leukocyte activation and enhancing endogenous antioxidant activity.
引用
收藏
页码:450 / 456
页数:7
相关论文
共 50 条
  • [31] Protective effect of crepidiastrum denticulatum extract pretreatment against hepatic ischemia-reperfusion injury in mice
    Jang, Hyuk Jai
    Park, Chun Soo
    Lee, Hwa Mee
    Lee, Ji Hwan
    Oh, Mee Young
    Kim, Seong Soo
    Noh, Ju Won
    Lee, Hee Ju
    TRANSPLANTATION, 2016, 100 (07) : S508 - S508
  • [32] Protective effect of Ulvan (sulfated polysaccharide) Pretreatment against Hepatic Ischemia-Reperfusion injury in Mice
    Jang, Hyuk Jai
    Park, Cheon Soo
    Lee, Hwa Mi
    You, Sang Guan
    TRANSPLANTATION, 2018, 102 : S712 - S712
  • [33] Protective Effect of MESNA (2-Mercaptoethane Sulfonate) Against Hepatic Ischemia/Reperfusion Injury in Rats
    Goksel Sener
    Ozer Sehirli
    Feriha Ercan
    Serap Sirvancı
    Nursal Gedik
    Ayhan Kacmaz
    Surgery Today, 2005, 35 : 575 - 580
  • [34] Protective effect of MESNA (2-mercaptoethane sulfonate) against hepatic ischemia/reperfusion injury in rats
    Sener, G
    Sehirli, O
    Ercan, F
    Sirvanci, S
    Gedik, N
    Kacmaz, A
    SURGERY TODAY, 2005, 35 (07) : 575 - 580
  • [35] Protective Effect of Citric Acid Against Hepatic Ischemia Reperfusion Injury in Sprague-Dawley Rats
    Kim, Su Kang
    Kang, Sang Wook
    Jin, Seul A.
    Ban, Ju Yeon
    Hong, Seoung-Jin
    Park, Min-Su
    TRANSPLANTATION PROCEEDINGS, 2019, 51 (08) : 2823 - 2827
  • [36] Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
    Yu, Shuna
    Zheng, Jie
    Jiang, Zhengchen
    Shi, Caixing
    Li, Jin
    Du, Xiaodong
    Wang, Hailiang
    Jiang, Jiying
    Wang, Xin
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (09) : 17680 - 17693
  • [38] Protective effect of matrine against cerebral ischemia reperfusion injury in rats
    Peng, Xiaochun
    Zhang, Kun
    Ma, Hongying
    1678, Trade Science Inc, 126,Prasheel Park,Sanjay Raj Farm House,Nr. Saurashtra Unive, Rajkot, Gujarat, 360 005, India (08): : 1678 - 1681
  • [39] PROTECTIVE EFFECT OF PENTOXIFYLLINE AGAINST LIVER ISCHEMIA REPERFUSION INJURY IN RATS
    XING, XP
    CURRIN, RT
    THURMAN, RG
    LEMASTERS, JJ
    GASTROENTEROLOGY, 1993, 104 (04) : A970 - A970
  • [40] Protective effect of tetramethylpyrazine against intestinal ischemia-reperfusion injury
    Toth, S.
    Varga, J.
    Pekarova, T.
    Toth, S., Sr.
    Vesela, J.
    HISTOPATHOLOGY, 2012, 61 : 95 - 95