Imaging of biological tissues with pixel-level analysis of second-order susceptibility

被引：10

作者：

Hu, Po-Sheng ^{[2
]}

Ghazaryan, Ara ^{[1
]}

Hovhannisyan, Vladimir ^{[1
]}

Chen, Shean-Jen ^{[2
]}

Chen, Yang-Fang ^{[1
]}

Kim, Chang-Seok ^{[3
]}

Tsai, Tsung-Hua ^{[4
]}

Dong, Chen-Yuan ^{[1
,3
,5
]}

机构：

[1] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan

[2] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 70101, Taiwan

[3] Pusan Natl Univ, Dept Cognomechatron Engn, Pusan, South Korea

[4] Far Eastern Mem Hosp, Dept Dermatol, New Taipei City 220, Taiwan

[5] Natl Taiwan Univ, Ctr Quantum Sci & Engnstr, Taipei 106, Taiwan

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2013年 / 18卷 / 03期

关键词：

second-order susceptibility; second harmonic generation; collagen; polarization-resolved microscopy; 2ND-HARMONIC GENERATION; IN-VIVO; EXCITATION MICROSCOPY; OPTICAL MICROSCOPY; STRATUM-CORNEUM; COLLAGEN; POLARIZATION; FLUORESCENCE; DISCRIMINATION; MYOSIN;

D O I：

10.1117/1.JBO.18.3.031102

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We discuss the recent advances in the development and applications of second-order susceptibility as a contrast mechanism in optical microscopy for biological tissues. We review nonlinear optical methods and approaches for differentiation of tissue structures and discrimination of normal and pathological skin tissues, which have been demonstrated for the potential use in clinical diagnosis. In addition, the potential of second-order susceptibility imaging, encompassing applications in differentiating various types of collagen molecules for clinical diagnosis, is demonstrated. Finally, we discuss future development and application of this technique. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

引用

页数：9

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