Effect of increasing levels of bioflavonoids in broiler feed on plasma anti-oxidative potential, lipid metabolites, and fatty acid composition of meat

This study was conducted to investigate the supplemental effects of purified bioflavonoids (genistein and hesperidin), as potential alternatives to plant/herbs or synthetic antioxidants, individually and in combination for fatty acid profile, lipid metabolites, and antioxidant status of broilers. Three hundred sixty 1-d-old broilers were divided into 6 treatment groups: control (basal diet), G5 (5 mg of genistein per kg of feed), and H20 (20 mg hesperidin per kg of feed), whereas the other 3 groups were supplemented with a mixture of genistein and hesperidin (20% genistein + 80% hesperidin) having a dosage of 5 mg.kg(-1) (GH5), 10 mg.kg(-1) (GH10), and 20 mg.kg(-1) (GH20), respectively. Broilers were slaughtered at 42 d, and breast muscle, liver, and blood samples were collected. A dose-dependent increase (P < 0.05) was observed for plasma antioxidant parameters, including total antioxidant capacity, malondialdehyde production, and total superoxide dismutase activity. Cholesterol and triglyceride contents were found to decrease (P < 0.05) in serum and breast muscle. The proportion of total polyunsaturated fatty acids and the ratio of n-6 to n-3 fatty acids and polyunsaturated fatty acids to saturated fatty acids in breast muscles was significantly improved (P < 0.05) by increasing levels of dietary bioflavonoids. The current results implied that dietary bioflavonoids genistein and hesperidin could positively improve the fatty acid and lipid metabolite profile of broiler breast meat in a dose-dependent fashion. Thus, bioflavonoids could be a feasible alternative of antioxidant plants/herbs and synthetic feed additives for the production of healthier chicken meat.