Cultured rat microglia express functional β-chemokine receptors

被引：102
|
作者：
Boddeke, EWGM
Meigel, I
Frentzel, S
Gourmala, NG
Harrison, JK
Buttini, M
Spleiss, O
Gebicke-Härter, P
机构：
[1] Univ Groningen, Dept Med Physiol, NL-9712 KZ Groningen, Netherlands
[2] Novartis Pharma, NS Res, CH-4002 Basel, Switzerland
[3] Univ Florida, Dept Neurosci, Gainesville, FL 32610 USA
[4] Univ Calif San Francisco, Gladstone Mol Neurobiol Program, San Francisco, CA 94141 USA
[5] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94141 USA
[6] Univ Freiburg, Dept Psychiat, D-79104 Freiburg, Germany
来源：
JOURNAL OF NEUROIMMUNOLOGY | 1999年 / 98卷 / 02期
关键词：
chemokines; signal transduction; lipopolysaccharide; neuroimmunology;
D O I：
10.1016/S0165-5728(99)00096-X
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We have investigated the functional expression of the beta-chemokine receptors CCR1 to 5 in cultured rat microglia. RT-PCR analysis revealed constitutive expression of CCR1, CCR2 and CCR5 mRNA. The beta-chemokines MCP-1 (1-30 nM) as well as RANTES and MIP-1 alpha (100-1000 nM) evoked calcium transients in control and LPS-treated microglia. Whereas, the response to MCP-1 was dependent on extracellular calcium the response to RANTES was not. The effect of MCP-1 but not that of RANTES was inhibited by the calcium-induced calcium release inhibitor ryanodine. Calcium responses to MCP-1- and RANTES were observed in distinct populations of microglia. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：176 / 184
页数：9
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