Quantification of S-adenosyl methionine in microbial cell extracts

被引：3
|
作者：
Krämer, JO [1 ]
Heinzle, E [1 ]
Wittmann, C [1 ]
机构：
[1] Univ Saarland, D-66023 Saarbrucken, Germany
来源：
BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 02期
关键词：
AdoMet; C-1; metabolism; GC-MS; intracellular; methionine; metabolome; SAM;
D O I：
10.1007/s10529-005-4947-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A sensitive method for quantification of S-adenosyl methionine (SAM) in microbial cell extracts was developed and applied to Corynebacterium glutamicum. The method is based on SAM being completely hydrolyzed into O-18-homoserine when extracted in boiling (H2O)-O-18 and thus can be clearly distinguished by GC-MS analysis from naturally labeled homoserine present in the cell extract. Additional quantification of the total homoserine pool, representing both SAM and homoserine, via HPLC allows separate determination of both metabolites.
引用
收藏
页码：69 / 71
页数：3
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