Laboratory methods to study African swine fever virus

被引：53
|
作者：
de Leon, Patricia [1 ]
Bustos, Maria J. [1 ]
Carrascosa, Angel L. [1 ]
机构：
[1] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
来源：
VIRUS RESEARCH | 2013年 / 173卷 / 01期
关键词：
African swine fever virus (ASFV); Production; Susceptibility; Target cells; Purification; Titration; Deletion mutants; Swine macrophages; WSL; ASF vaccine; CELL-LINES; VIRULENCE DETERMINANT; EFFICIENT REPLICATION; ALVEOLAR MACROPHAGES; GENETIC MANIPULATION; NUCLEAR-FACTOR; DOMESTIC PIGS; HEMADSORPTION; EXPRESSION; ACTIVATION;
D O I：
10.1016/j.virusres.2012.09.013
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We summarize findings of comparative studies in different cells cultures susceptible to ASFV infection, through the analysis of virus components and infectious virus particles production, as alternative means to grow field and laboratory ASFV strains. We also provide different methods to assay the infectivity of ASFV samples and to purify the infective virus particles. Finally we describe the general strategy to construct virus deletion mutants that can be engineered to obtain attenuated ASFV strains suitable for vaccine approaches. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：168 / 179
页数：12
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