Quantification of Breast Cancer Cells in Peripheral Blood Samples by Real-Time RT-PCR

被引：0
|
作者：
Zebisch, Michael [1 ]
Koelbl, Alexandra C. [1 ]
Schindlbeck, Christian [2 ]
Neugebauer, Julia [1 ]
Heublein, Sabine [1 ]
Ilmer, Matthias [3 ]
Rack, Brigitte [1 ]
Friese, Klaus [1 ]
Jeschke, Udo [1 ]
Andergassen, Ulrich [1 ]
机构：
[1] Univ Munich, Dept Obstet & Gynaecol, D-80337 Munich, Germany
[2] Clin Traunstein, Traunstein, Germany
[3] Univ Texas Houston, Dept Mol Pathol, Houston, TX USA
来源：
ANTICANCER RESEARCH | 2012年 / 32卷 / 12期
关键词：
Breast carcinoma; CTC; cytokeratin; MCF7; ZR-75-1; cells; CIRCULATING TUMOR-CELLS; BONE-MARROW; EXPRESSION; SURVIVAL; CARCINOMAS; DIAGNOSIS; MARKER; MMP-13; GENE;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers. Materials and Methods: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. RNA was isolated from the samples and transcribed into cDNA. TagMan real-time PCR for cytokeratins 8, 18 and 19 was carried out and was correlated to that of 18S. Results: Cytokeratin gene expression increased in all samples, when as few as 10 tumour cells were added. In the CAMA-1 cell line, the increase was even greater the more cells were added. Conclusion: By this methodology, cells from mammary carcinoma cell lines can be detected in blood samples. Its benefit will be validated in samples from patients with breast cancer.
引用
收藏
页码：5387 / 5391
页数：5
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