Quantification of Breast Cancer Cells in Peripheral Blood Samples by Real-Time RT-PCR

被引：0

作者：

Zebisch, Michael ^{[1
]}

Koelbl, Alexandra C. ^{[1
]}

Schindlbeck, Christian ^{[2
]}

Neugebauer, Julia ^{[1
]}

Heublein, Sabine ^{[1
]}

Ilmer, Matthias ^{[3
]}

Rack, Brigitte ^{[1
]}

Friese, Klaus ^{[1
]}

Jeschke, Udo ^{[1
]}

Andergassen, Ulrich ^{[1
]}

机构：

[1] Univ Munich, Dept Obstet & Gynaecol, D-80337 Munich, Germany

[2] Clin Traunstein, Traunstein, Germany

[3] Univ Texas Houston, Dept Mol Pathol, Houston, TX USA

来源：

ANTICANCER RESEARCH | 2012年 / 32卷 / 12期

关键词：

Breast carcinoma; CTC; cytokeratin; MCF7; ZR-75-1; cells; CIRCULATING TUMOR-CELLS; BONE-MARROW; EXPRESSION; SURVIVAL; CARCINOMAS; DIAGNOSIS; MARKER; MMP-13; GENE;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers. Materials and Methods: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. RNA was isolated from the samples and transcribed into cDNA. TagMan real-time PCR for cytokeratins 8, 18 and 19 was carried out and was correlated to that of 18S. Results: Cytokeratin gene expression increased in all samples, when as few as 10 tumour cells were added. In the CAMA-1 cell line, the increase was even greater the more cells were added. Conclusion: By this methodology, cells from mammary carcinoma cell lines can be detected in blood samples. Its benefit will be validated in samples from patients with breast cancer.

引用

页码：5387 / 5391

页数：5

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