This study was designed to assess the effects of road traffic noise and frogs' croaking on the objective and subjective quality of sleep in a laboratory. The subjects were seven male students aged 19-21 years. They were exposed to recorded road traffic noise and frogs' croaking, with 49.6 and 49.5 dB(A) L-Aeq, and 71.2 and 56.1 dB(A) L-Amax, respectively. The background noise in the experimental room was 31.0 dB(A) L-Aeq. The sleep EEG was recorded according to standard methods. The sleep polygraphic parameters examined were the percentage of sleep stage relative to the total sleep time (%S1, %S2, %S(3 + 4), %SREM, %MT), total sleep time, sleep onset latency, and awakening during sleep in minutes and sleep efficiency. A structured sleep rating questionnaire (OSA), was administered to the subjects after they awakened. The %S2 increased and the %SREM decreased during exposure to road traffic noise. However, no significant effect of exposure to frogs' croaking was observed on any of the polygraphic sleep parameters. The subjective quality of sleep was degraded more by exposure to road traffic noise than that to frogs' croaking. (C) 2002 Academic Press.