Immunoinformatic Analysis of T- and B-Cell Epitopes for SARS-CoV-2 Vaccine Design

被引:45
|
作者
Wang, Dongliang [1 ]
Mai, Jinhui [1 ]
Zhou, Wenfeng [1 ]
Yu, Wanting [1 ,2 ]
Zhan, Yang [1 ]
Wan, Naidong [1 ]
Epstein, Neal D. [3 ]
Yang, Yi [1 ]
机构
[1] Hunan Agr Univ, Coll Vet Med, Res Ctr Reverse Vaccinol RCRV, Hunan Prov Key Lab Prot Engn Anim Vaccines,Lab Fu, Changsha 410128, Peoples R China
[2] Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100871, Peoples R China
[3] NHLBI, Cell & Dev Biol Ctr, NIH, Bldg 10, Bethesda, MD 20892 USA
关键词
SARS-CoV-2; S protein; B-cell and T-cell epitopes; vaccine; SYNDROME SARS CORONAVIRUS; SPIKE PROTEIN; FUNCTIONAL RECEPTOR; STRUCTURAL BASIS; S2; DOMAIN; IDENTIFICATION; NEUTRALIZATION; RESPONSES; ENTRY; COV;
D O I
10.3390/vaccines8030355
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Currently, there is limited knowledge about the immunological profiles of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). We used computer-based immunoinformatic analysis and the newly resolved 3-dimensional (3D) structures of the SARS-CoV-2 S trimeric protein, together with analyses of the immunogenic profiles of SARS-CoV, to anticipate potential B-cell and T-cell epitopes of the SARS-CoV-2 S protein for vaccine design, particularly for peptide-driven vaccine design and serological diagnosis. Nine conserved linear B-cell epitopes and multiple discontinuous B-cell epitopes composed of 69 residues on the surface of the SARS-CoV-2 trimeric S protein were predicted to be highly antigenic. We found that the SARS-CoV-2 S protein has a different antigenic profile than that of the SARS-CoV S protein due to the variations in their primary and 3D structures. Importantly, SARS-CoV-2 may exploit an immune evasion mechanism through two point mutations in the critical and conserved linear neutralization epitope (overlap with fusion peptide) around a sparsely glycosylated area. These mutations lead to a significant decrease in the antigenicity of this epitope in the SARS-CoV-2 S protein. In addition, 62 T-cell epitopes in the SARS-CoV-2 S protein were predicted in our study. The structure-based immunoinformatic analysis for the SARS-CoV-2 S protein in this study may improve vaccine design, diagnosis, and immunotherapy against the pandemic of COVID-19.
引用
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页码:1 / 15
页数:15
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