Quantitative Analyses of Forster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo-FRET) In DNA Scaffolds.

被引：8

作者：

Kashida, Hiromu ^{[1
]}

Kawai, Hayato ^{[1
]}

Azuma, Hidenori ^{[1
]}

Araki, Yasuyuki ^{[2
]}

Wada, Takehiko ^{[2
]}

Asanuma, Hiroyuki ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Dept Biomol Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan

[2] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japan

来源：

CHEMPHOTOCHEM | 2021年 / 5卷 / 02期

关键词：

DNA; energy transfer; energy migration; FRET; pyrene; ORIENTATION DEPENDENCE; NUCLEIC-ACID; HETERO-FRET; PROBE; FLUOROPHORES; INSERTION; CY3;

D O I：

10.1002/cptc.202000199

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Forster resonance energy transfer between identical chromophores (homo-FRET) has been difficult to analyze since neither emission intensity nor lifetime changes with the occurrence of homo-FRET. Herein we used a DNA scaffold to analyze homo-FRET between pyrene moieties. The DNA scaffold was modified with two pyrenes and a quencher, anthraquinone. Homo-FRET was detected by monitoring quenching of pyrene emission and the decrease in the fluorescence lifetime of pyrene. Homo-FRET efficiencies could be calculated by excluding effects of hetero-FRET. The experimentally determined efficiencies showed an excellent agreement with Forster theory. These results will inform design of novel molecular probes and light-harvesting antennae.

引用

页码：167 / 172

页数：6