Polymerase Chain Reaction Assay for Rapid, Sensitive Detection, and Identification of Colletotrichum gloeosporioides causing Greater Yam Anthracnose

被引：10

作者：

Raj, Mithun ^{[1
]}

Jeeva, M. L. ^{[1
]}

Hegde, V. ^{[1
]}

Vidyadharan, Pravi ^{[1
]}

Archana, P. V. ^{[1
]}

Senthil alias Sankar, M. ^{[1
]}

Nath, S. Vishnu ^{[1
]}

机构：

[1] Cent Tuber Crops Res Inst, Div Crop Protect, Thiruvananthapuram 695017, Kerala, India

来源：

MOLECULAR BIOTECHNOLOGY | 2012年 / 52卷 / 03期

关键词：

Dioscorea alata; Molecular diagnosis; rDNA; Nested PCR; Glomerella cingulata; Phytopathogen; Die back; PCR-BASED DETECTION; PHYTOPHTHORA-NICOTIANAE; DIFFERENTIATION; DNA;

D O I：

10.1007/s12033-012-9496-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anthracnose caused by Colletotrichum gloeosporioides is an economically important disease which affects greater yam (Dioscorea alata L.) worldwide. Apart from airborne conidia, the pathogen propagules surviving in soil and planting material are the major sources of inoculum. A nested PCR assay has been developed for specific detection of C. gloeosporioides in soil and planting material. In conventional (single-round) PCR, the limit of detection was 20 pg, whereas in nested PCR the detection limit increased to 0.2 pg of DNA. The primers designed were found to be highly specific and could be used for accurate identification of the pathogen up to species level. The protocol was standardized for detection of the pathogen in artificially and naturally infected field samples.

引用

页码：277 / 284

页数：8

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