Bacterial inclusion bodies: making gold from waste

被引：125
|
作者：
Garcia-Fruitos, Elena [1 ,2 ,3 ]
Vazquez, Esther [1 ,2 ,3 ]
Diez-Gil, Cesar [1 ,4 ]
Luis Corchero, Jose [1 ,2 ,3 ]
Seras-Franzoso, Joaquin [1 ,2 ,3 ]
Ratera, Imma [1 ,4 ]
Veciana, Jaume [1 ,4 ]
Villaverde, Antonio [1 ,2 ,3 ]
机构：
[1] CIBER BBN, Barcelona 08193, Spain
[2] Univ Autonoma Barcelona, Inst Biotechnol & Biomed, E-08193 Barcelona, Spain
[3] Univ Autonoma Barcelona, Dept Genet & Microbiol, E-08193 Barcelona, Spain
[4] CSIC, Inst Ciencia Mat Barcelona, Dept Mol Nanosci & Organ Mat, E-08193 Barcelona, Spain
来源：
TRENDS IN BIOTECHNOLOGY | 2012年 / 30卷 / 02期
关键词：
ESCHERICHIA-COLI; PROTEIN AGGREGATION; ADHESION; NANOMATERIAL; SOLUBILITY; QUALITY;
D O I：
10.1016/j.tibtech.2011.09.003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Many protein species produced in recombinant bacteria aggregate as insoluble protein clusters named inclusion bodies (IBs). IBs are discarded from further processing or are eventually used as a pure protein source for in vitro refolding. Although usually considered as waste byproducts of protein production, recent insights into the physiology of recombinant bacteria and the molecular architecture of lBs have revealed that these protein particles are unexpected functional materials. In this Opinion article, we present the relevant mechanical properties of lBs and discuss the ways in which they can be explored as biocompatible nanostructured materials, mainly, but not exclusively, in biocatalysis and tissue engineering.
引用
收藏
页码：65 / 70
页数：6
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