Isolation and identification of two novel umami and umami-enhancing peptides from peanut hydrolysate by consecutive chromatography and MALDI-TOF/TOF MS

被引：123
|
作者：
Su, Guowan [1 ]
Cui, Chun [1 ]
Zheng, Lin [1 ]
Yan, Bao [2 ]
Ren, Jiaoyan [1 ]
Zhao, Mouming [1 ]
机构：
[1] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China
[2] Chinese Acad Sci, Key Lab Plant Resource Conservat & Sustainable Ut, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
来源：
FOOD CHEMISTRY | 2012年 / 135卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Peanut hydrolysate; Umami; Umami-enhancing effect; Ultrafiltration; MALDI-TOF/TOF MS; ENZYMATIC HYDROLYSATE; INHIBITORY PEPTIDES; TASTE; ACID; PURIFICATION;
D O I：
10.1016/j.foodchem.2012.04.130
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Peanut hydrolysate produced by crude protease extract from Aspergillus oryzae HN 3.042 was found to elicit intense umami and umami-enhancing effect. Taste profiles, amino acid and organic acid composition of peanut hydrolysate and its separation fractions by ultrafiltration were evaluated. The results revealed that peanut hydrolysate was mainly low molecular weight compounds. Fractions of 1-3 kDa and below 1 kDa prominently contributed to the umami taste and umami-enhancing effect of the peanut hydrolysate. The two fractions were further purified, using gel filtration chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC), in combination with sensory evaluation, to obtain a umami peptide and umami-enhancing peptide. The active peptides were identified as Ser-Ser-Arg-Asn-Glu-Gln-Ser-Arg (SSRNEQSR. 963.9 Da) and Glu-Gly-Ser-Glu-Ala-Pro-Asp-Gly-Ser-Ser-Arg (EGSEAPDGSSR, 1091.1 Da), by MALDI-TOF/TOF MS, respectively. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：479 / 485
页数：7
相关论文
共 50 条
  • [41] Target-directed isolation and identification of a serum lectin from lamprey (Lampetra japonica) by chromatographys and MALDI-TOF/TOF
    Yinglun Han
    Meng Gou
    Xiaoping Song
    Tao Song
    Biyue Shi
    Yue Pang
    Qingwei Li
    Acta Oceanologica Sinica, 2018, 37 : 113 - 116
  • [42] Rapid identification of microorganisms directly from positive blood cultures by MALDI-TOF MS
    Oviano, Marina
    ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, 2019, 37 (05): : 287 - 289
  • [43] An alternative for urine cultures: Direct identification of uropathogens from urine by MALDI-TOF MS
    Ilki, Arzu Aksit
    Ozsoy, Sevim
    Gelmez, Gulsen
    Aksu, Burak
    Soyletir, Guner
    ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 2020, 67 (03) : 193 - 197
  • [44] MALDI-TOF MS simple and rapid tool for identification of bacteria isolated from the environment
    Stursa, Petr
    Junkova, Petra
    Strejcek, Michal
    Macek, Tomas
    Mackova, Martina
    LISTY CUKROVARNICKE A REPARSKE, 2010, 126 (11): : 412 - 413
  • [45] Identification of bacteria isolated from veterinary clinical specimens using MALDI-TOF MS
    Pavlovic, Melanie
    Wudy, Corinna
    Zeller-Peronnet, Veronique
    Maggipinto, Marzena
    Zimmermann, Pia
    Straubinger, Alix
    Iwobi, Azuka
    Maertlbauer, Erwin
    Busch, Ulrich
    Huber, Ingrid
    BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT, 2015, 128 (1-2): : 24 - 30
  • [46] Target-directed isolation and identification of a serum lectin from lamprey (Lampetra japonica) by chromatographys and MALDI-TOF/TOF
    Han Yinglun
    Gou Meng
    Song Xiaoping
    Song Tao
    Shi Biyue
    Pang Yue
    Li Qingwei
    ACTA OCEANOLOGICA SINICA, 2018, 37 (05) : 113 - 116
  • [47] MALDI-TOF MS analysis for detection of bovine coronavirus with tryptic peptides from viral proteins
    Hayashi, Katsuhiko
    Ohya, Kenji
    Yoshinari, Tomoya
    Hirose, Shouhei
    Shimizu, Souta
    Morita, Yuji
    Ohnishi, Takahiro
    Watanabe, Maiko
    Taharaguchi, Satoshi
    Mekata, Hirohisa
    Taniguchi, Takahide
    Hara-kudo, Yukiko
    JOURNAL OF MICROORGANISM CONTROL, 2024, 29 (04): : 143 - 151
  • [48] Comparison of two MALDI-TOF MS systems for the identification of clinically relevant anaerobic bacteria in Argentina
    Litterio, Mirta
    Castello, Liliana
    Venuta, Maria Elena
    Abel, Sofia
    Fernandez-Canigia, Liliana
    Legaria, Maria Cristina
    Rollet, Raquel
    Vaustat, Daniela
    Azula, Natalia
    Fox, Barbara
    Otero, Silvina
    Maldonado, Maria Laura
    Mangieri, Natalia Alejandra
    Rossetti, Maria Adelaida
    Predari, Silvia Carla
    Cejas, Daniela
    Barberis, Claudia
    REVISTA ARGENTINA DE MICROBIOLOGIA, 2024, 56 (01): : 33 - 61
  • [49] Comparison and optimization of two MALDI-TOF MS platforms for the identification of medically relevant yeast species
    M. A. Pence
    E. McElvania TeKippe
    M. A. Wallace
    C.-A. D. Burnham
    European Journal of Clinical Microbiology & Infectious Diseases, 2014, 33 : 1703 - 1712
  • [50] Improvement of mosquito identification by MALDI-TOF MS biotyping using protein signatures from two body parts
    Vega-Rua, Anubis
    Pages, Nonito
    Fontaine, Albin
    Nuccio, Christopher
    Hery, Lyza
    Goindin, Daniella
    Gustave, Joel
    Almeras, Lionel
    PARASITES & VECTORS, 2018, 11
12345