Toxic effects of acrylic acid on Clostridium propionicum and isolation of acrylic acid-tolerant mutants for production of acrylic acid

被引：9

作者：

Luo, Wei ^{[1
,2
,3
]}

Cai, Jin ^{[1
]}

Zhu, Linqi ^{[1
]}

Zhu, Xiangcheng ^{[1
]}

Huang, Lei ^{[1
]}

Xu, Zhinan ^{[1
]}

Cen, Peilin ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem & Biol Engn, Inst Biol Engn, Hangzhou 310027, Zhejiang, Peoples R China

[2] Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Wuxi, Peoples R China

[3] Jiangnan Univ, Sch Biotechnol, Wuxi, Peoples R China

来源：

ENGINEERING IN LIFE SCIENCES | 2012年 / 12卷 / 05期

关键词：

Acrylic acid; Acrylic acid tolerant mutants; Clostridium propionicum; Inhibition; ESCHERICHIA-COLI; ACETIC-ACID; PH; GROWTH; PROPIONIBACTERIA; THERMOACETICUM; FERMENTATION; EVOLUTION; LACTATE; ACETATE;

D O I：

10.1002/elsc.201100200

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This work presents a systematic investigation of the toxic effects of acrylic acid on the growth of Clostridium propionicum and the isolation of acrylic acid-tolerant mutants. The results suggest that addition of acrylic acid prolonged the lag phase of the fermentation and reduced the initial-specific growth rate, as well as the final cell concentration. Moreover, the toxic effect of acrylic acid was sensitive to the pH value. The minimal inhibition concentration of acrylic acid increased from 1.11 to 31.25 mM when the pH value rose from 5.8 to 7.4. In addition, the molar concentration ratio of products (acetic acid:propionic acid) was enhanced with the supplementation of acrylic acid. The highest ratio was 0.7:1 when acrylic acid was 20.83 mM at pH 7.4. Two acrylic acid-tolerant mutants were isolated, which could still grow at a high concentration (43.06 mM) of acrylic acid. These strains could be instrumental for improved bioproduction of acrylic acid.

引用

页码：567 / 573

页数：7

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