Cell-free fat extract accelerates diabetic wound healing in db/db mice

被引:3
|
作者
Wang, Xiangsheng [1 ]
Deng, Mingwu [1 ]
Yu, Ziyou [1 ]
Cai, Yizuo [1 ]
Liu, Wei [1 ,2 ]
Zhou, Guangdong [1 ,2 ]
Wang, Xiansong [1 ]
Cao, Yilin [1 ,2 ]
Li, Wei [1 ]
Zhang, Wenjie [1 ,2 ]
机构
[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med,Shanghai Key Lab Tissue Engn, Shanghai 200011, Peoples R China
[2] Natl Tissue Engn Ctr China, Shanghai 200041, Peoples R China
来源
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2020年 / 12卷 / 08期
基金
中国国家自然科学基金;
关键词
Fat extract; chronic wound healing; pro-angiogenesis; anti-inflammation; growth factors; cell-free therapy; STEM-CELLS; BIOLOGICAL CHARACTERISTICS; OXIDATIVE STRESS; STROMAL CELLS; ANGIOGENESIS; THERAPY; INFLAMMATION; SKIN;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Cell-free fat extract (CEFFE), the liquid fraction derived from fat tissues, is enriched with a variety of growth factors and possesses pro angiogenic, anti-apoptotic, and anti-oxidative properties. The aim of this study was to determine if CEFFE could accelerate chronic wound healing in mice with diabetes and investigate its underlying mechanisms. A model of circularfull-thickness wound (6 mm diameter) was produced in the central dorsal region of spontaneous type 2 diabetes mellitus db/db mice. The mice were divided to three groups depending on dosage of CEFFE administered for the study; high dose CEFFE group (CEFFEhigh; administered 2.5 ml/kg/day via subcutaneous injection for six days), low dose CEFFE group (CEFFElow; administered 2.5 ml/kg/day via subcutaneous injection for three days), and a control group receiving phosphate buffer solution. Wound closure was evaluated on day 3, 7,10, and 14 post-operation. Histological analyses, including hematoxylin-eosin staining and Masson's trichrome staining and immunohistological staining of anti-CD31 and anti-CD68, were also performed. Moreover, the effects of CEFFE on proliferation, migration, and tube formation of human immortal keratinocyte cells (HaCaT) and human vascular endothelial cells (HUVEC) were tested in vitro. The results showed that the local injection of CEFFE significantly accelerated wound healing in mice with diabetes. CEFFE improved re-epithelization and collagen secretion, promoted angiogenesis, and inhibited inflammatory macrophage infiltration in vivo. CEFFE also promoted HaCaT proliferation and migration and enhanced tubular formation in cultured HUVEC. It was concluded that CEFFE accelerates wound healing through pro-angiogenic and anti-inflammatory activities.
引用
收藏
页码:4216 / 4227
页数:12
相关论文
共 50 条
  • [21] Topical application of yeast extract accelerates the wound healing of diabetic mice
    Crowe, MJ
    McNeill, RB
    Schlemm, DJ
    Greenhalgh, DG
    Keller, SJ
    JOURNAL OF BURN CARE & REHABILITATION, 1999, 20 (02): : 155 - 162
  • [22] STIMULATORY EFFECTS OF HYDROCOLLOID SHEETS COMPOSED OF CHITIN/CHITOSAN, FUCOIDAN, AND ALGINATE AS WOUND DRESSING ON WOUND HEALING IN DIABETIC DB/DB MICE
    Yanagibayashi, Satoshi
    Ishihara, Masayuki
    WOUND REPAIR AND REGENERATION, 2012, 20 (01) : A9 - A9
  • [23] Wound closure rate does not correlate with hyperglycemia or body weight in an excisional diabetic wound healing model using db/db mice
    Troudsale, Rhonda K.
    Jacobs, Sharone'
    Freeby, Matthew J.
    Simhaee, David A.
    Wu, June
    Joyce, Lustbader W.
    DIABETES, 2006, 55 : A246 - A246
  • [24] Topical Administration of a Marine Oil Rich in Pro-Resolving Lipid Mediators Accelerates Wound Healing in Diabetic db/db Mice through Angiogenesis and Macrophage Polarization
    Ontoria-Oviedo, Imelda
    Amaro-Prellezo, Elena
    Castellano, Delia
    Venegas-Venegas, Elena
    Gonzalez-Santos, Fernando
    Ruiz-Sauri, Amparo
    Pelacho, Beatriz
    Prosper, Felipe
    Perez del Caz, Maria Dolores
    Sepulveda, Pilar
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (17)
  • [25] Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice
    Li, Di
    Li, Lin
    Xu, Teng
    Wang, Tianxing
    Ren, Jinwei
    Liu, Xinran
    Li, Yong
    MARINE DRUGS, 2018, 16 (01):
  • [26] Impaired wound healing in diabetic (db/db) mice is associatedwith dysregulation of thrombospondin-1 and-2 expression
    Volk, S. W.
    Hakkinen, L.
    Herdrich, B. J.
    Zhang, L.
    Shitaye, H.
    Hankenson, K. D.
    Liechty, K. W.
    WOUND REPAIR AND REGENERATION, 2008, 16 (02) : A15 - A15
  • [27] Delayed wound healing in diabetic (db/db) mice with Pseudomonas aeruginosa biofilm challenge: a model for the study of chronic wounds
    Zhao, Ge
    Hochwalt, Phillip C.
    Usui, Marcia L.
    Underwood, Robert A.
    Singh, Pradeep K.
    James, Garth A.
    Stewart, Philip S.
    Fleckman, Philip
    Olerud, John E.
    WOUND REPAIR AND REGENERATION, 2010, 18 (05) : 467 - 477
  • [28] Therapeutic Potential of Exosomes Derived from Diabetic Adipose Stem Cells in Cutaneous Wound Healing of db/db Mice
    Hsu, Hsiang-Hao
    Wang, Aline Yen Ling
    Loh, Charles Yuen Yung
    Pai, Ashwin Alke
    Kao, Huang-Kai
    PHARMACEUTICS, 2022, 14 (06)
  • [29] Phytosterol Extract Decreases the Oxidative Damage in the Brains of Diabetic db/db Mice
    Salgado-Roman, Juan Manuel
    Loza-Cornejo, Sofia
    Hernandez-Soto, Rosa
    Hernandez-Maldonado, Jose Alfredo
    Marquez-Villalobos, Fatima Airam
    Perez-Vazquez, Victoriano
    Franco-Robles, Elena
    Ramirez-Emiliano, Joel
    METABOLIC SYNDROME AND RELATED DISORDERS, 2021, 19 (05) : 305 - 311
  • [30] WOUND TREATMENT WITH ANGIOGENIC PROVISIONAL MATRIX ENHANCES WOUND NEOVASCULARIZATION AND IMPROVES HEALING IN DB/DB MICE
    Balaji, Swathi
    Sheikh, Abdul Q.
    Vaikunth, Sachin S.
    Parvadia, Jignesh K.
    Lim, Foong-Yen
    Crombleholme, Timothy M.
    Narmoneva, Daria A.
    WOUND REPAIR AND REGENERATION, 2009, 17 (02) : A15 - A15