Multi-color polymer pen lithography for oligonucleotide arrays

被引:26
|
作者
Kumar, R. [1 ,2 ,3 ,4 ]
Weigel, S. [5 ]
Meyer, R. [5 ]
Niemeyer, C. M. [5 ]
Fuchs, H. [1 ,2 ,3 ,4 ]
Hirtz, M. [1 ,2 ]
机构
[1] Karlsruhe Inst Technol, Inst Nanotechnol INT, D-76344 Eggenstein Leopoldshafen, Germany
[2] Karlsruhe Inst Technol, Karlsruhe Nano Micro Facil KNMF, D-76344 Eggenstein Leopoldshafen, Germany
[3] Univ Munster, Inst Phys, D-48 Munster, Germany
[4] Univ Munster, Ctr Nanotechnol CeNTech, Munster 48, Germany
[5] Karlsruhe Inst Technol, Inst Biol Interfaces IBG 1, D-76344 Eggenstein Leopoldshafen, Germany
关键词
PROTEIN ARRAYS; FABRICATION; MICROARRAYS; BIOLOGY;
D O I
10.1039/c6cc07087f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Multi-color patterning by polymer pen lithography (PPL) was used to fabricate covalently immobilized fluorophore and oligonucleotide arrays with up to five different components. The oligonucleotide arrays offer a virtually unlimited inventory of orthogonal binding tags for self-assembly of proteins as demonstrated by use of the arrays to monitor cell-protein interactions of MCF7 cells.
引用
收藏
页码:12310 / 12313
页数:4
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