Genome and Transcriptome of Clostridium phytofermentans, Catalyst for the Direct Conversion of Plant Feedstocks to Fuels

被引：18

作者：

Petit, Elsa ^{[1
]}

Coppi, Maddalena V. ^{[1
]}

Hayes, James C. ^{[2
,3
]}

Tolonen, Andrew C. ^{[4
]}

Warnick, Thomas ^{[1
]}

Latouf, William G. ^{[1
,3
]}

Amisano, Danielle ^{[1
]}

Biddle, Amy ^{[1
,3
]}

Mukherjee, Supratim ^{[1
,3
]}

Ivanova, Natalia ^{[5
]}

Lykidis, Athanassios ^{[5
]}

Land, Miriam ^{[6
]}

Hauser, Loren ^{[6
]}

Kyrpides, Nikos ^{[5
]}

Henrissat, Bernard ^{[7
]}

Lau, Joanne ^{[1
]}

Schnell, Danny J. ^{[8
]}

Church, George M. ^{[9
]}

Leschine, Susan B. ^{[1
,3
,10
]}

Blanchard, Jeffrey L. ^{[1
,2
,3
,11
,12
]}

机构：

[1] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA

[2] Univ Massachusetts, Grad Program Mol & Cellular Biol, Amherst, MA 01003 USA

[3] Univ Massachusetts, Inst Cellular Engn, Amherst, MA 01003 USA

[4] CNRS, Commissariat Energie Atom & Energies Alternat CEA, Unite Mixte Rech UMR 8030, Evry, France

[5] Joint Genome Inst, Dept Energy DOE, Genome Biol Program, Prod Genom Facil, Walnut Creek, CA USA

[6] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN USA

[7] CNRS, Unite Mixte Rech UMR 6098, Architecture & Fonct Macromol Biol, Marseille, France

[8] Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA

[9] Harvard Univ, Sch Med, Dept Genet, Boston, MA USA

[10] Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA

[11] Univ Massachusetts, Grad Program Organismal & Evolutionary Biol, Amherst, MA 01003 USA

[12] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA

来源：

PLOS ONE | 2015年 / 10卷 / 06期

基金：

美国国家科学基金会;

关键词：

SEQUENCE ALIGNMENT; GENE-EXPRESSION; FAMILY; IDENTIFICATION; CELLULOSE; ETHANOL; KEGG; ENDOGLUCANASE; FERREDOXIN; RESOURCE;

D O I：

10.1371/journal.pone.0118285

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Clostridium phytofermentans was isolated from forest soil and is distinguished by its capacity to directly ferment plant cell wall polysaccharides into ethanol as the primary product, suggesting that it possesses unusual catabolic pathways. The objective of the present study was to understand the molecular mechanisms of biomass conversion to ethanol in a single organism, Clostridium phytofermentans, by analyzing its complete genome and transcriptome during growth on plant carbohydrates. The saccharolytic versatility of C. phytofermentans is reflected in a diversity of genes encoding ATP-binding cassette sugar transporters and glycoside hydrolases, many of which may have been acquired through horizontal gene transfer. These genes are frequently organized as operons that may be controlled individually by the many transcriptional regulators identified in the genome. Preferential ethanol production may be due to high levels of expression of multiple ethanol dehydrogenases and additional pathways maximizing ethanol yield. The genome also encodes three different proteinaceous bacterial microcompartments with the capacity to compartmentalize pathways that divert fermentation intermediates to various products. These characteristics make C. phytofermentans an attractive resource for improving the efficiency and speed of biomass conversion to biofuels.

引用

页数：18

共 21 条

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Sung, Young Joon
Lee, Jeong Seop
Yoon, Hong Ki
Ko, Hyunjin
Sim, Sang Jun
SYSTEMS MICROBIOLOGY AND BIOMANUFACTURING, 2021, 1 (01): : 90 - 99

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