Actinomyces wuliandei sp. nov., Corynebacterium liangguodongii sp. nov., Corynebacterium yudongzhengii sp. nov. and Oceanobacillus zhaokaii sp. nov., isolated from faeces of Tibetan antelope in the Qinghai-Tibet plateau of China

Eight Gram-stain-positive, rod-shaped bacterial strains were isolated from faeces of Tibetan antelopes on the Tibet-Qinghai Plateau of China. Genomic sequence analysis showed that the strains belong to the genera Actinomyces (strains 299(T) and 340), Corynebacterium (strains 2184(T), 2185, 2183(T) and 2189) and Oceanobacillus (strains 160(T) and 143), respectively, with a percentage of similarity for the 16S rRNA gene under the species threshold of 98.7% except for strains 160(T) and 143 with Oceanobacillus arenosus CAU 1183(T) (98.8%). The genome sizes (and genomic G+C contents) were 3.1 Mb (49.4%), 2.5 Mb (64.9%), 2.4 Mb (66.1%) and 4.1 Mb (37.1%) for the type strains 299(T), 2183(T), 2184(T) and 160(T), respectively. Two sets of the overall genome relatedness index values between our isolates and their corresponding closely related species were under species thresholds (95% for average nucleotide identity, and 70% for digital DNA-DNA hybridization). These results, together with deeper genotypic, genomic, phenotypic and biochemical analyses, indicate that these eight isolates should be classified as representing four novel species. Strain 299(T) (=CGMCC 1.16320(T)=JCM 33611(T)) is proposed as representing Actinomyces wuliandei sp. nov.; strain 2184(T) (=CGMCC 1.16417(T)=DSM 106203(T)) is proposed as representing Corynebacterium liangguodongii sp. nov.; strain 2183(T) (=CGMCC 1.16416(T)=DSM 106264(T)) is proposed as representing Corynebacterium yudongzhengii sp. nov.; and strain 160(T) (=CGMCC 1.16367(T)=DSM 106186(T)) is proposed as representing Oceanobacillus zhaokaii sp. nov.