Structural and biological properties of Cucumber mosaic virus particles carrying hepatitis C virus-derived epitopes

被引：18
|
作者：
Nuzzaci, M. [1 ]
Bochicchio, I. [2 ]
De Stradis, A. [3 ]
Vitti, A. [1 ]
Natilla, A. [1 ]
Piazzolla, P. [1 ]
Tamburro, A. M. [2 ]
机构：
[1] Univ Basilicata, Dept Biol Plant Protect & Agrobiotechnol, I-85100 Potenza, Italy
[2] Univ Basilicata, Dept Chem, I-85100 Potenza, Italy
[3] Sect Bari, CNR, Plant Virol Inst, I-70125 Bari, Italy
来源：
JOURNAL OF VIROLOGICAL METHODS | 2009年 / 155卷 / 02期
关键词：
Chimeric CMV particles; Circular dichroism; Electron microscopy; Structural properties; HCV; RNA;
D O I：
10.1016/j.jviromet.2008.10.005
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the CMV-D strain. The CP gene was then engineered to express one or two copies of a synthetic peptide derived from many hypervariable region 1 (HVR1) sequences of the Hepatitis C virus (HCV) envelope protein E2 (the so-called R9 mimotope). Study of the symptoms pattern displayed in tobacco by these chimeric CMV particles, together with determination of their structural characteristics, assessed by circular dichroism (CD) spectroscopy and electron microscopy, revealed a possible relationship between the biological behavior and the structural properties of virus components. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：118 / 121
页数：4
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