Staining Undecalcified Bone Sections a Modified Technique for an Improved Visualization of Synthetic Bone Substitutes

被引：0
|
作者：
Mayr-Wohlfart, U. [2 ]
Ravalli, G. [3 ]
Guenther, K. P. [4 ]
Kessler, S. [1 ]
机构：
[1] Sindelfingen Dist Hosp, Dept Orthopaed, D-71065 Sindelfingen, Germany
[2] Inst Transfus Med & Immunogenet, Ulm, Germany
[3] Univ Ulm, Dept Orthopaed, Ulm, Germany
[4] Univ Dresden, Dept Orthopaed, Dresden, Germany
来源：
ACTA CHIRURGIAE ORTHOPAEDICAE ET TRAUMATOLOGIAE CECHOSLOVACA | 2008年 / 75卷 / 06期
关键词：
bone histology; hard tissue histology; light mircroscopy; biomaterials; synthetic implants; staining; undecalcified sections;
D O I：
暂无
中图分类号：
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];
学科分类号：
摘要：
We describe a detailed embedding procedure for large bone spicemens in methyl methacrylate and a new staining method by which thin sections (appr 100 um) of undecalcified bones with synthetic implants can be coloured. Different staining effects were obtained which greatly facilitated the evaluation of sections with bone, new forming bone and especially remnants of synthetic implants. The identification and quantification of the latter is difficult in common staining techniques. A detailed embedding - staining - mounting procedure is proposed.
引用
收藏
页码：443 / 445
页数：3
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