An Optical Trap Combined with Three-Color FRET

被引：30
|
作者：
Lee, Sanghwa [1 ,2 ]
Hohng, Sungchul [1 ,2 ]
机构：
[1] Seoul Natl Univ, Dept Biophys & Chem Biol, Dept Phys & Astron, Seoul 151747, South Korea
[2] Seoul Natl Univ, Natl Ctr Creat Res Initiat, Seoul 151747, South Korea
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 49期
基金：
新加坡国家研究基金会;
关键词：
SINGLE-MOLECULE FLUORESCENCE; MAGNETIC TWEEZERS; FORCE SPECTROSCOPY; HOLLIDAY JUNCTIONS; ENERGY-TRANSFER; DNA; RESOLUTION; DYNAMICS;
D O I：
10.1021/ja408767p
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We developed a hybrid technique combining optical tweezers and single-molecule three-color fluorescence resonance energy transfer (FRET). In demonstrative experiments, we observed the force-sensitive correlated motion of three helical arms of a Holliday junction and identified the independent unfolding/folding dynamics of two DNA hairpins of the same length. With 3 times the number of observable elements of single-molecule FRET, this new instrument will enable the measurement of the complex, multidimensional effects of mechanical forces in various biomolecular systems, such as RNA and proteins.
引用
收藏
页码：18260 / 18263
页数：4
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