CHEMICALLY MODIFIED C SILICA-BASED COLUMNS: UNIVERSAL STATIONARY PHASES FOR HPLC SEPARATIONS

被引：0
|
作者：
Soukup, Jan [1 ]
Jandera, Pavel [1 ]
机构：
[1] Dept Analyt Chem, Pardubice 53210, Czech Republic
来源：
CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS | 2012年
关键词：
C silica-based stationary phases; aqueous normal-phase LC; dual retention mechanism; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; MOBILE-PHASE; TEMPERATURE; MECHANISM; ACIDS;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We tested the retention of phenolic acids in full mobile phase (acetonitrile/water containing buffer) composition range on two chemically modified hydrosilated (C silica-based) columns in order to show versatility of those stationary phases. Cogent UDC cholesterol (TM) and Cogent bidentate C18 (TM) columns show significant dual reversed-phase/normal-phase retention behavior, hence those stationary phases can be used both in aqueous normal-phase (ANP) and in reversed-phase (RP) modes. The effect of the aqueous acetate buffer concentration on retention factors of phenolic acids over the full mobile phase composition range, including both ANP and RP mechanisms, can be described by a four-parameter equation for dual-retention mechanism.
引用
收藏
页码：53 / 58
页数：6
相关论文
共 50 条
  • [31] NEW ASPECTS CONCERNING THE STABILITY OF SILICA-BASED HPLC SEPARATION PHASES
    FALKENHAGEN, J
    DIETRICH, PG
    AMERICAN LABORATORY, 1994, 26 (14) : 48 - &
  • [32] SEPARATIONS ON CAPILLARY COLUMNS WITH LIQUID-CRYSTAL STATIONARY PHASES
    LITKOVSKAYA, LL
    FEDYANIN, AA
    KIRSH, SI
    JOURNAL OF ANALYTICAL CHEMISTRY OF THE USSR, 1991, 46 (01): : 146 - 148
  • [33] Separation of dietary folates by gradient reversed-phase HPLC:: Comparison of alternative and conventional silica-based stationary phases
    Johansson, M
    Jastrebova, J
    Grahn, A
    Jägerstad, M
    CHROMATOGRAPHIA, 2005, 62 (1-2) : 33 - 40
  • [34] Separation of Dietary Folates by Gradient Reversed-Phase HPLC: Comparison of Alternative and Conventional Silica-Based Stationary Phases
    M. Johansson
    J. Jastrebova
    A. Grahn
    M. Jägerstad
    Chromatographia, 2005, 62 : 33 - 40
  • [35] THE SYNTHESIS AND CHARACTERIZATION OF CHEMICALLY BONDED SILICA-BASED ZWITTERION-EXCHANGERS FOR HPLC
    YU, LW
    FLOYD, TR
    HARTWICK, RA
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1986, 24 (05) : 177 - 182
  • [36] Comparison of Different Silica-Based Imidazolium Stationary Phases for LC in Separation of Alkaloids
    Wentao Bi
    Kyung Ho Row
    Chromatographia, 2010, 71 : 25 - 30
  • [37] Comparison of zirconia- and silica-based reversed stationary phases for separation of enkephalins
    Soukupová, K
    Krafková, E
    Suchánková, J
    Tesarová, E
    JOURNAL OF CHROMATOGRAPHY A, 2005, 1087 (1-2) : 104 - 111
  • [38] Development of silica-based stationary phases for high-performance liquid chromatography
    Hongdeng Qiu
    Xiaojing Liang
    Min Sun
    Shengxiang Jiang
    Analytical and Bioanalytical Chemistry, 2011, 399 : 3307 - 3322
  • [39] Development of silica-based stationary phases for high-performance liquid chromatography
    Qiu, Hongdeng
    Liang, Xiaojing
    Sun, Min
    Jiang, Shengxiang
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 399 (10) : 3307 - 3322
  • [40] Comparison of Different Silica-Based Imidazolium Stationary Phases for LC in Separation of Alkaloids
    Bi, Wentao
    Row, Kyung Ho
    CHROMATOGRAPHIA, 2010, 71 (1-2) : 25 - 30
12345