In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

被引：17
|
作者：
Foster, Aaron E. [1 ,2 ]
Kwon, Sunkuk [3 ]
Ke, Shi [3 ]
Lu, An [1 ,2 ]
Eldin, Karen [4 ]
Sevick-Muraca, Eva [3 ]
Rooney, Cliona M. [1 ,2 ]
机构：
[1] Texas Childrens Hosp, Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA
[2] Methodist Hosp, Houston, TX 77030 USA
[3] Baylor Coll Med, Div Mol Imaging, Dept Radiol, Frensley Ctr Imaging Res, Houston, TX 77030 USA
[4] Texas Childrens Hosp, Dept Pathol, Houston, TX 77030 USA
来源：
APPLIED OPTICS | 2008年 / 47卷 / 31期
关键词：
D O I：
10.1364/AO.47.005944
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW Labeling of Epstein-Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDYe800CW tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together. these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal Model and may have potential as a short-term reporter molecule for human immunotherapy studies. (C) 2008 Optical Society of America
引用
收藏
页码：5944 / 5952
页数：9
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