Cranial bone regeneration via BMP-2 encoding mesenchymal stem cells

被引：29
|
作者：
Vural, Altugan Cahit [1 ]
Odabas, Sedat [2 ]
Korkusuz, Petek [3 ]
Saglam, Atiye Seda Yar [4 ]
Bilgic, Elif [3 ]
Cavusoglu, Tarik [1 ]
Piskin, Erhan [5 ,6 ]
Vargel, Ibrahim [7 ]
机构：
[1] Kirikkale Univ, Fac Med, Dept Plast & Aesthet Surg, Kirikkale, Turkey
[2] Ankara Univ, Dept Chem, Fac Sci, Ankara, Turkey
[3] Hacettepe Univ, Dept Histol & Embryol, Fac Med, Ankara, Turkey
[4] Gazi Univ, Dept Mol Biol & Genet, Fac Med, Ankara, Turkey
[5] Hacettepe Univ, Dept Chem Engn, Ankara, Turkey
[6] Hacettepe Univ, Bioengn Div, Ctr Bioengn Biyomedtek, Ankara, Turkey
[7] Hacettepe Univ, Fac Med, Dept Plast & Aesthet Surg, Ankara, Turkey
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2017年 / 45卷 / 03期
关键词：
Bone morphogenetic protein-2; cranial bone regeneration; critical size defect; mesenchymal stem cell; transfection; MORPHOGENETIC PROTEIN-2; GROWTH-FACTOR; IN-VIVO; SCAFFOLDS; CRYOGELS; DEFECTS;
D O I：
10.3109/21691401.2016.1160918
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cranial bone repair and regeneration via tissue engineering principles has attracted a great deal of interest from researchers during last decade. Here, within this study, 6mm critical-sized bone defect regeneration via genetically modified mesenchymal stem cells (MSC) were monitored up to 4 months. Cranial bone repair and new bone formations were evaluated by histological staining and real time PCR analysis in five different groups including autograft and bone morphogenetic protein-2 (BMP-2) transfected MSC groups. Results presented here indicate a proper cranial regeneration in autograft groups and a prospering regeneration for hBMP-2 encoding mesenchymal stem cells.
引用
收藏
页码：544 / 550
页数：7
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