Simultaneous Detection of SERS and Fluorescence Using a Single Excitation for Microbead-Based Analysis

被引:4
|
作者
Lee, Sa Ram [1 ]
Jeon, Chang Su [2 ]
Hwang, Inseong [2 ]
Chung, Taek Dong [2 ]
Kim, Hee Chan [3 ,4 ]
机构
[1] Seoul Natl Univ, Interdisciplinary Program Biomed Engn Major, Seoul 151742, South Korea
[2] Seoul Natl Univ, Dept Chem, Seoul 151742, South Korea
[3] Seoul Natl Univ, Dept Biomed Engn, Coll Med, Seoul 110744, South Korea
[4] Seoul Natl Univ, Inst Med & Biol Engn, Med Res Ctr, Seoul 110744, South Korea
基金
新加坡国家研究基金会;
关键词
Multiplex Analysis; Surface-Enhanced Raman Spectroscopy (SERS); Fluorescence; Simultaneous Detection; Silver Microbeads; ENCODING MICROCARRIERS; BEADS;
D O I
10.1166/jbn.2013.1517
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
We demonstrate simultaneous detection of surface-enhanced Raman scattering (SERS) and fluorescence signals from a silver microbead. For the dual signal generation, silver microbeads with a diameter of 15 mu m were functionalized with benzenethiol (BT) as a Raman tag and a cardiac troponin I (cTnI) antibody on their surface. SERS and fluorescence signals were obtained using a single argon laser source with 488 nm wavelength. The SERS signals from Raman tag can be used as identification indices for decoding a particular microbead, while the fluorescence signals provide the information about molecular interactions with a specific biomarker. With simultaneous detection of SERS and fluorescence signals using single excitation on the functionalized microbeads, we successfully showed the possibility of a simple barcoding strategy for multiplex analysis using suspension arrays.
引用
收藏
页码:1241 / 1244
页数:4
相关论文
共 50 条
  • [21] Analyzing protein-protein interactions in rare cells using microbead-based single-molecule pulldown assay
    Zhao, Qirui
    Shen, Yusheng
    Li, Xiaofen
    Tian, Fang
    Yu, Xiaojie
    Yobas, Levent
    Park, Hyokeun
    Duan, Yuanyuan
    Huang, Pingbo
    LAB ON A CHIP, 2021, 21 (16) : 3137 - 3149
  • [22] Development of a multiplex microbead-based immunoassay for quantitative analysis of mouse immune checkpoint proteins
    Lie, Wen-Rong
    Kornmeier, Christine
    Williams, Sasha
    Pfeifer, Justin
    Banerjee, Munmun
    Hoberg, James
    JOURNAL OF IMMUNOLOGY, 2022, 208 (01):
  • [23] Sensitive and selective viral DNA detection assay via microbead-based rolling circle amplification
    Schopf, Eric
    Fischer, Nicholas O.
    Chen, Yong
    Tok, Jeffrey B. -H.
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2008, 18 (22) : 5871 - 5874
  • [24] Microbead-based affinity chromatography chip using RNA aptamer modified with photocleavable linker
    Cho, S
    Lee, SH
    Chung, WJ
    Kim, YK
    Lee, YS
    Kim, BG
    ELECTROPHORESIS, 2004, 25 (21-22) : 3730 - 3739
  • [25] Dye-Sensitized Solar Cells Using Mesocarbon Microbead-Based Counter Electrodes
    Hsieh, Chien-Te
    Yang, Bing-Hao
    Chen, Wei-Yu
    INTERNATIONAL JOURNAL OF PHOTOENERGY, 2012, 2012
  • [26] Simultaneous detection of multiple microRNAs based on fluorescence resonance energy transfer under a single excitation wavelength
    Liu, Lin
    He, Jia Hui
    Wu, Xiao Qiao
    Liu, Jia Jun
    Lv, Wen Yi
    Huang, Cheng Zhi
    Liu, Hui
    Li, Chun Mei
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2024, 322
  • [27] Magnetic Encoding Plasmonic Janus Microbead-Based Suspension Array for High Sensitivity Multiplex Analysis
    Sheng, Tao
    Xie, Zhuoying
    Liu, Panmiao
    Chen, Jialun
    Chen, Shan
    Ding, Hailong
    Deng, Jingzhe
    Yuan, Yi
    Deng, Dawei
    ADVANCED MATERIALS INTERFACES, 2018, 5 (19):
  • [28] Single microbead-based fluorescent aptasensor (SMFA) for direct isolation and in situ quantification of exosomes from plasma
    Wang, Fangfang
    Zhang, Yi
    Chen, Desheng
    Zhang, Zhuoqi
    Li, Zhengping
    ANALYST, 2021, 146 (10) : 3346 - 3351
  • [29] Magnetic microbead-based enzyme-linked immunoassay for detection of Schistosoma japonicum antibody in human serum
    Liu, Zhenshi
    Zhang, Li
    Yang, Hai
    Zhu, Yanhong
    Jin, Wei
    Song, Qian
    Yang, Xiangliang
    ANALYTICAL BIOCHEMISTRY, 2010, 404 (02) : 127 - 134
  • [30] Multiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels
    Yu, Hye-Weon
    Kim, In S.
    Niessner, Reinhard
    Knopp, Dietmar
    ANALYTICA CHIMICA ACTA, 2012, 750 : 191 - 198