Real-time observation of single-molecule fluorescence in microdroplet streams

被引：38
|
作者：
Barnes, MD
Lermer, N
Kung, CK
Whitten, WB
Ramsey, JM
Hill, SC
机构：
[1] Chem. and Analyt. Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6142
[2] Army Research Laboratory, Adelphi, MD 20783-1155
来源：
OPTICS LETTERS | 1997年 / 22卷 / 16期
关键词：
D O I：
10.1364/OL.22.001265
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximate to 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3-12-mu m diameter) with <1% shot-to-shot size fluctuations and sensitivity comparable with that of droplet levitation techniques with at least 10(3) higher analysis rates. Applications to the study of single-molecule microcavity effects and stimulated emission are discussed.
引用
收藏
页码：1265 / 1267
页数：3
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