Ionic liquids microemulsions: the key to Candida antarctica lipase B superactivity

被引：57
|
作者：
Ventura, Sonia P. M. [1 ]
Santos, Luisa D. F. [1 ]
Saraiva, Jorge A.
Coutinho, Joao A. P. [1 ,2 ]
机构：
[1] Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro, Portugal
[2] Univ Aveiro, Dept Quim, QOPNA, P-3810193 Aveiro, Portugal
来源：
GREEN CHEMISTRY | 2012年 / 14卷 / 06期
关键词：
AQUEOUS-SOLUTIONS; ENZYME-ACTIVITY; CATALYZED TRANSESTERIFICATION; HOFMEISTER SERIES; MICELLE FORMATION; SELF-AGGREGATION; ORGANIC-SOLVENTS; STABILITY; BIOCATALYSIS; ESTERIFICATION;
D O I：
10.1039/c2gc35197h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The activity of the commercial enzyme Candida antarctica lipase B (CaLB), in aqueous solutions of the ionic liquid 1-decyl-3-methylimidazolium chloride, [C(10)mim]Cl, at pH 7.0 was investigated. The relative enzyme activity (Act(IL)/Act(Bf)) for ionic liquid molar concentrations ranging from 0.000-0.150 mol L-1 show an increase up to six fold in Act(IL)/Act(Bf), with the ionic liquid molar concentration. This phenomenon is shown to be related with the formation of micelles originated by the self-aggregation of [C(10)mim]Cl. The enzyme activation energy is not affected by the ionic liquid presence, suggesting the absence of structural changes in the enzyme induced by the ionic liquid. It is here demonstrated, for the first time, that it is possible to significantly increase the activity of an enzyme simply by using aqueous solutions of ionic liquids.
引用
收藏
页码：1620 / 1625
页数：6
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