Enhancing adhesion of yeast brewery strains to chamotte carriers through aminosilane surface modification

被引:16
|
作者
Berlowska, Joanna [1 ]
Kregiel, Dorota [1 ]
Ambroziak, Wojciech [1 ]
机构
[1] Tech Univ Lodz, Inst Fermentat Technol & Microbiol, PL-90924 Lodz, Poland
来源
关键词
Proadhesive agents; Aminosilane; Chamotte; Yeast immobilization; CONTINUOUS BEER FERMENTATION; SACCHAROMYCES-CEREVISIAE; STAINLESS-STEEL; CELLS; IMMOBILIZATION; TEMPERATURE; ALGINATE;
D O I
10.1007/s11274-013-1294-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The adhesion of cells to solid supports is described as surface-dependent, being largely determined by the properties of the surface. In this study, ceramic surfaces modified using different organosilanes were tested for proadhesive properties using industrial brewery yeast strains in different physiological states. Eight brewing strains were tested: bottom-fermenting Saccharomyces pastorianus and top-fermenting Saccharomyces cerevisiae. To determine adhesion efficiency light microscopy, scanning electron microscopy and the fluorymetric method were used. Modification of chamotte carriers by 3-(3-anino-2-hydroxy-1-propoxy) propyldimethoxysilane and 3-(N, N-dimethyl-N-2-hydroxyethyl) ammonium propyldimethoxysilane groups increased their biomass load significantly.
引用
收藏
页码:1307 / 1316
页数:10
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