Platelet rich plasma associated or not to autogenous bone graft on the reparation of experimental bone defects in the rabbits cranium

The present study evaluated autogenous platelet rich plasma's (PRP) influence on the reparation process of filter bone defects made oil rabbit's skull, associated or not to autogenous bone graft (EOE). Defect I received PRP only defect II received 3mg of EOE only: defect III received EOE associated to PRP: defect IV was left to heal naturally, serving as control. After each surgery the animals were randomly divided into three groups that were euthanized at 30, 60 and 90 days. In the mesoscopic evaluation bone ingrowth started from the defect's borders to the center and from the bottom to the surface for all observation times on the control (VI) and PRP only (I) groups. In the groups treated with EOE only (II) and EOE associated to PRP (III) new bone wets observed in the center of the defects. Radiographic analysis showed greater central radiopacity for groups treated with EOE only (II) and EOE associated to PRP (III) at all observation times. Microscopically III till, group treated with FOE associated to PRP (III) at 30 days the graft was indistinguishable from new bone present on the border of the defect, associated to it moderate quantity of a very vascularized and cellular fibrous connective tissue. This tissue showed till extracelular eosinophilic amorphous foamy material, associated to ant inflammatory process constituted by lymphocytes and in less number by macrophages and multinucleated giant cells that may have negatively. influenced early bone formation. At 60 and 90 days occasional spots of lymphocytic inflammation were observed. Both treatments, PRP associated or not to EOE, were similar for the bone ingrowth at the final time of observation; the graft used alone determined early bone reparation and thromboplastine used for the platelet gel formation incited a foreign body-like reaction that acted negatively on the initial reparation.