A new method for improving the accuracy of miRNA detection with NaYF4:Yb,Er upconversion nanoparticles

被引:149
|
作者
Mao, Lanlan [1 ]
Lu, Zhuoxuan [1 ]
He, Nongyue [1 ,3 ]
Zhang, Liming [1 ]
Deng, Yan [1 ]
Duan, Deming [2 ]
机构
[1] Hunan Univ Technol, Econ Forest Cultivat & Utilizat 2011 Collaborat I, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China
[2] Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China
[3] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
microRNAs detection; NaYF4:Yb; Er; reference standard; base stacking hybridization; BOVINE SERUM-ALBUMIN; MICRORNA DETECTION; IN-SITU; DNA; LUMINESCENCE; NANOCRYSTALS; ENHANCEMENT; RNA;
D O I
10.1007/s11426-016-0021-0
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
MicroRNAs (miRNAs) are small noncoding RNAs, which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis. So far, many miRNAs detection methods require polymerase chain reaction (PCR) amplification following reverse transcription of miRNAs. These processes are complicated and time-consuming. In this work, we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF4:Yb,Er upconversion nanoparticles. In this method, the fluorescence of NaYF4:Yb,Er upconversion nanoparticles were functionalized as a reference standard, which can improve the accuracy of miRNA detection. On the basis of these findings, we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.
引用
收藏
页码:157 / 162
页数:6
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