Two-photon laser scanning microscopy integrated with light stimulus system

被引：0

作者：

Xu, Xin ^{[1
,2
]}

Jin, Xingjie ^{[1
]}

Gao, Feng ^{[1
]}

Shi, Guohua ^{[1
]}

机构：

[1] Chinese Acad Sci, Jiangsu Key Lab Med Opt, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China

[2] Univ Elect Sci & Technol China, Sch Optoelect Sci & Engn, Chengdu 610052, Sichuan, Peoples R China

来源：

FIFTH SYMPOSIUM ON NOVEL OPTOELECTRONIC DETECTION TECHNOLOGY AND APPLICATION | 2019年 / 11023卷

关键词：

Two-photon microscopy; light stimulus; light intensity modulation; laser scanning microscopy;

D O I：

10.1117/12.2521012

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate a home-made two-photon laser scanning microscopy (TPLSM) with a light stimulus system. In this system, the femtosecond pulses are produced by a picosecond fiber laser with pulse width compression. A laser diode serves as stimulus, which modulated by an AOM and coupled into the same light path of a femtosecond laser. The control signal of AOM and trigger signal of two scanning mirrors are synchronized by a FPGA board. With modulating the intensity of CW laser at precise point in one scanning frame, any pattern of light stimulus can be delivered to the sample in real time.

引用

页数：5

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