Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury

被引：86
|
作者：
Hashimoto, Manabu [1 ]
Ishii, Kunihiro [2 ]
Nakamura, Yasuko [1 ]
Watabe, Kazuhiko [3 ]
Kohsaka, Shinichi [1 ]
Akazawa, Chihiro [1 ,2 ]
机构：
[1] NCNP, Natl Inst Neurosci, Dept Neurochem, Kodaira, Tokyo 1878502, Japan
[2] Tokyo Med & Dent Univ, Grad Sch Hlth & Sci, Dept Biochem & Biophys, Tokyo, Japan
[3] Tokyo Metropolitan Inst Neurosci, Tokyo, Japan
来源：
JOURNAL OF NEUROCHEMISTRY | 2008年 / 107卷 / 04期
关键词：
brain-derived neurotrophic factor; nerve injury; real-time polymerase chain reaction; Schwann cells; sciatic nerve; sonic hedgehog;
D O I：
10.1111/j.1471-4159.2008.05666.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The physiological roles of sonic hedgehog (Shh) have been intensively characterized in development of various organs. However, their functions in adult tissues have not been fully elucidated. We investigated the expression and the potential function of Shh in crush-injured adult rat sciatic nerves. The Shh expression was up-regulated in Schwann cells adjacent to the injured site. The time-course analyses of various neurotrophic factors revealed the up-regulation of Shh mRNA followed by that of brain-derived neurotrophic factor (BDNF) mRNA. The continuous administration of cyclopamine, a hedgehog signal inhibitor, to the injured site suppressed the increase of BDNF expression and deteriorated the survival of motor neurons in lumbar spinal cord. Treatment of exogenous Shh in cultured Schwann cells enhanced the BDNF expression. The BDNF promoter activity (exon I and II) was increased in IMS32 cells co-transfected with Shh and its receptor Smoothened. These findings imply that the up-regulated expression of Shh in Schwann cells may play an important role in injured motor neurons through the induction of BDNF.
引用
收藏
页码：918 / 927
页数：10
相关论文
共 50 条
  • [21] Neuroprotective Effect of Nypa fruticans Wurmb by Suppressing TRPV1 Following Sciatic Nerve Crush Injury in a Rat
    Kang, Mi-Sun
    Lee, Gil-Hyun
    Choi, Go-Eun
    Yoon, Hae-Gyung
    Hyun, Kyung-Yae
    NUTRIENTS, 2020, 12 (09) : 1 - 14
  • [22] EXPRESSION OF TENASCIN ISOFORMS ARE SELECTIVELY UP-REGULATED FOLLOWING AORTIC BALLOON INJURY
    SHARIFI, BG
    LAFLEUR, DW
    SCHWARTZ, SM
    FORRESTER, JS
    FAGIN, JA
    FASEB JOURNAL, 1995, 9 (03): : A611 - A611
  • [23] The miR-17/92 Polycistron Is Up-regulated in Sonic Hedgehog-Driven Medulloblastomas and Induced by N-myc in Sonic Hedgehog-Treated Cerebellar Neural Precursors
    Northcott, Paul A.
    Fernandez-L, Africa
    Hagan, John P.
    Ellison, David W.
    Grajkowska, Wesia
    Gillespie, Yancey
    Grundy, Richard
    Van Meter, Timothy
    Rutka, James T.
    Croce, Carlo M.
    Kenney, Anna Marie
    Taylor, Michael D.
    CANCER RESEARCH, 2009, 69 (08) : 3249 - 3255
  • [24] Resveratrol regulates the recovery of rat sciatic nerve crush injury by promoting the autophagy of Schwann cells
    Zhang, Jiayi
    Ren, Jingyan
    Liu, Yang
    Huang, Dongxu
    Lu, Laijin
    LIFE SCIENCES, 2020, 256
  • [25] INTERLEUKIN-6 PRODUCTION BY SCHWANN-CELLS AND INDUCTION IN SCIATIC-NERVE INJURY
    BOLIN, LM
    VERITY, AN
    SILVER, JE
    SHOOTER, EM
    ABRAMS, JS
    JOURNAL OF NEUROCHEMISTRY, 1995, 64 (02) : 850 - 858
  • [26] Spatiotemporal expression of KHSRP modulates Schwann cells and neuronal differentiation after sciatic nerve injury
    Zhu, Xiaojian
    Yao, Li
    Yang, Xiaojing
    Sun, Huiqing
    Guo, Aisong
    Li, Aihong
    Yang, Huilin
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2014, 48 : 1 - 10
  • [27] Platelet-rich plasma gel in combination with Schwann cells for repair of sciatic nerve injury
    Fagang Ye
    NeuralRegenerationResearch, 2012, 7 (29) : 2286 - 2292
  • [28] Up-Regulation of CD146 in Schwann Cells Following Peripheral Nerve Injury Modulates Schwann Cell Function in Regeneration
    Shen, Yinying
    Zhu, Jun
    Liu, Qianyan
    Ding, Shiyan
    Dun, Xinpeng
    He, Jianghong
    FRONTIERS IN CELLULAR NEUROSCIENCE, 2021, 15
  • [29] Platelet-rich plasma gel in combination with Schwann cells for repair of sciatic nerve injury
    Ye, Fagang
    Li, Haiyan
    Qiao, Guangxi
    Chen, Feng
    Tao, Hao
    Ji, Aiyu
    Hu, Yanling
    NEURAL REGENERATION RESEARCH, 2012, 7 (29) : 2286 - 2292
  • [30] MECHANISM OF SONIC HEDGEHOG ACTIVATION IN INTESTINAL EPITHELIAL CELLS FOLLOWING IONIZING RADIATION INJURY
    Lakha, Rabina
    Orzechowska-Licari, Emilia J.
    Wu, Zhi Jian
    Bialkowska, Agnieszka B.
    Yang, Vincent W.
    GASTROENTEROLOGY, 2024, 166 (05) : S236 - S236
12345