Developments in cryo-electron tomography for in situ structural analysis

被引：20

作者：

Dubrovsky, Anna ^{[1
]}

Sorrentino, Simona ^{[1
]}

Harapin, Jan ^{[1
]}

Sapra, K. Tanuj ^{[1
]}

Medalia, Ohad ^{[1
,2
,3
]}

机构：

[1] Univ Zurich, Dept Biochem, CH-8057 Zurich, Switzerland

[2] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2015年 / 581卷

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

Cryo-electron tomography; Cryo-focused-ion beam; Direct electron detector; Phase plate; NUCLEAR-PORE COMPLEX; BEAM-INDUCED MOTION; PHASE-CONTRAST; ELECTRON TOMOGRAPHY; MOLECULAR ARCHITECTURE; 3-DIMENSIONAL STRUCTURE; BIOLOGICAL SPECIMENS; CELLULAR PROCESSES; PROTEIN COMPLEXES; EUKARYOTIC CELLS;

D O I：

10.1016/j.abb.2015.04.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：78 / 85

页数：8

共 50 条

[31] In situ cryo-electron tomography at the nucleus-cytoskeleton interface
Hutchings, Joshua
Shaikh, Farhaz
Villa, Elizabeth
BIOPHYSICAL JOURNAL, 2023, 122 (03) : 408A - 409A
[32] Quantitative Cryo-Electron Tomography
Navarro, Paula P.
FRONTIERS IN MOLECULAR BIOSCIENCES, 2022, 9
[33] 3D Structural Analysis of Flagella/Cilia by Cryo-Electron Tomography
Bui, Khanh Huy
Ishikawa, Takashi
CILIA, PT A, 2013, 524 : 305 - 323
[34] Structural Analysis of HIV-1 Maturation Using Cryo-Electron Tomography
de Marco, Alex
Mueller, Barbara
Glass, Baerbel
Riches, James D.
Kraeusslich, Hans-Georg
Briggs, John A. G.
PLOS PATHOGENS, 2010, 6 (11)
[35] Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging
Schur, Florian K. M.
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2019, 58 : 1 - 9
[36] Cellular and Structural Studies of Eukaryotic Cells by Cryo-Electron Tomography
Weber, Miriam Sarah
Wojtynek, Matthias
Medalia, Ohad
CELLS, 2019, 8 (01)
[37] Charting Cellular Landscapes in Molecular Detail by in Situ Cryo-Electron Tomography
Plitzko, Juergen
Mahamid, Julia
Engel, Benjamin D.
Albert, Sahradha
Schaffer, Miroslava
Arnold, Jan
Fukuda, Yoshiyuki
Danev, Radostin
Baumeister, Wolfgang
BIOPHYSICAL JOURNAL, 2016, 110 (03) : 2A - 2A
[38] In situ cryo-electron tomography and subtomogram averaging of intraflagellar transport trains
Jordan, Mareike A.
Pigino, Gaia
THREE-DIMENSIONAL ELECTRON MICROSCOPY, 2019, 152 : 179 - 195
[39] Solving Structure in Situ using Cryo-Electron Tomography and Correlative Methods
Briggs, John A. G.
BIOPHYSICAL JOURNAL, 2013, 104 (02) : 3A - 3A
[40] Cryo-Electron Tomography: The Resolution Revolution and a Surge of In Situ Virological Discoveries
Hong, Ye
Song, Yutong
Zhang, Zheyuan
Li, Sai
ANNUAL REVIEW OF BIOPHYSICS, 2023, 52 : 339 - 360

← 1 2 3 4 5 →