Real-time 3D single-molecule localization using experimental point spread functions

被引：190

作者：

Li, Yiming ^{[1
]}

Mund, Markus ^{[1
]}

Hoess, Philipp ^{[1
]}

Deschamps, Joran ^{[1
]}

Matti, Ulf ^{[1
]}

Nijmeijer, Bianca ^{[1
]}

Sabinina, Vilma Jimenez ^{[1
]}

Ellenberg, Jan ^{[1
]}

Schoen, Ingmar ^{[2
]}

Ries, Jonas ^{[1
]}

机构：

[1] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

[2] Royal Coll Surgeons Ireland, Mol & Cellular Therapeut, Dublin, Ireland

来源：

NATURE METHODS | 2018年 / 15卷 / 05期

基金：

欧洲研究理事会;

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; SUPERRESOLUTION MICROSCOPY; FLUORESCENCE MICROSCOPY; PARTICLE-TRACKING; RESOLUTION; ABERRATIONS; ALGORITHMS; NANOSCOPY; ACCURACY; PROBES;

D O I：

10.1038/nmeth.4661

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is compatible with any PSF engineering approach. We used this method to image cellular structures and attained unprecedented image quality for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D super-resolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.

引用

页码：367 / +

页数：7

共 50 条

[21] Accelerating 3D single-molecule localization microscopy using blind sparse inpainting
Gaire, Sunil Kumar
Wang, Yanhua
Zhang, Hao F.
Liang, Dong
Ying, Leslie
JOURNAL OF BIOMEDICAL OPTICS, 2021, 26 (02)
[22] vLUME: 3D virtual reality for single-molecule localization microscopy
Alexander Spark
Alexandre Kitching
Daniel Esteban-Ferrer
Anoushka Handa
Alexander R. Carr
Lisa-Maria Needham
Aleks Ponjavic
Ana Mafalda Santos
James McColl
Christophe Leterrier
Simon J. Davis
Ricardo Henriques
Steven F. Lee
Nature Methods, 2020, 17 : 1097 - 1099
[23] vLUME: 3D virtual reality for single-molecule localization microscopy
Spark, Alexander
Kitching, Alexandre
Esteban-Ferrer, Daniel
Handa, Anoushka
Carr, Alexander R.
Needham, Lisa-Maria
Ponjavic, Aleks
Santos, Ana Mafalda
McColl, James
Leterrier, Christophe
Davis, Simon J.
Henriques, Ricardo
Lee, Steven F.
NATURE METHODS, 2020, 17 (11) : 1097 - +
[24] The indoor real-time 3D localization algorithm using UWB
Li, Xuehong
Yang, Shuhua
2015 INTERNATIONAL CONFERENCE ON ADVANCED MECHATRONIC SYSTEMS (ICAMECHS), 2015, : 337 - 342
[25] Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization
Elias Amselem
Bo Broadwater
Tora Hävermark
Magnus Johansson
Johan Elf
Nature Communications, 14
[26] Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization
Amselem, Elias
Broadwater, Bo
Haevermark, Tora
Johansson, Magnus
Elf, Johan
NATURE COMMUNICATIONS, 2023, 14 (01)
[27] Plasmonic Assemblies for Real-Time Single-Molecule Biosensing
Armstrong, Rachel E.
Horacek, Matej
Zijlstra, Peter
SMALL, 2020, 16 (52)
[28] Real-time single-molecule imaging of quantum interference
Thomas Juffmann
Adriana Milic
Michael Müllneritsch
Peter Asenbaum
Alexander Tsukernik
Jens Tüxen
Marcel Mayor
Ori Cheshnovsky
Markus Arndt
Nature Nanotechnology, 2012, 7 (5) : 297 - 300
[29] Real-time single-molecule imaging of quantum interference
Juffmann, Thomas
Milic, Adriana
Muellneritsch, Michael
Asenbaum, Peter
Tsukernik, Alexander
Tuexen, Jens
Mayor, Marcel
Cheshnovsky, Ori
Arndt, Markus
NATURE NANOTECHNOLOGY, 2012, 7 (05) : 296 - 299
[30] Real-Time Single-Molecule Kinetics of Trypsin Proteolysis
Li, Jiangwei
Yeung, Edward S.
ANALYTICAL CHEMISTRY, 2008, 80 (22) : 8509 - 8513

← 1 2 3 4 5 →