Isolation, cloning, and expression of a new murine zinc finger encoding gene

被引:12
|
作者
Prost, JF [1 ]
Nègre, D [1 ]
Cornet-Javaux, F [1 ]
Cortay, JC [1 ]
Cozzone, AJ [1 ]
Herbage, D [1 ]
Mallein-Gerin, F [1 ]
机构
[1] Inst Biol & Chim Prot, CNRS UPR 412, F-69367 Lyon 07, France
关键词
mouse; cartilage; cDNA sequence; zinc finger motif; LeR domain;
D O I
10.1016/S0167-4781(99)00157-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
With the aim of identifying genes involved in cartilage differentiation, we have used a subtractive hybridization strategy with cDNAs from a chondrocytic cell line (MC615) and mRNAs from a mesenchymal precursor cell line (10T1/2). We have isolated a cDNA clone representing a novel mouse gene. The predicted 368-amino acid protein, designated ZF-12, contains four C2H2-type zinc finger motifs and one region homologous to the LeR domain, a finger-associated structural domain. ZF-12 mRNAs are expressed during embryonic development and in different organs in adult, including rib cartilage. These data suggest that ZF-12, might play an important role not only in cartilage differentiation, but also in basic cellular processes. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:278 / 283
页数:6
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