Localization of the neurokinin 1 receptor on a subset of substance P-positive and isolectin B4-negative dorsal root ganglion neurons of the rat

被引：17
|
作者：
von Banchet, GS [1 ]
Schaible, HG [1 ]
机构：
[1] Univ Jena, Inst Physiol, D-07740 Jena, Germany
来源：
NEUROSCIENCE LETTERS | 1999年 / 274卷 / 03期
关键词：
NK1; receptor; substance P; isolectin B4; colocalisation; dorsal root ganglion; rat;
D O I：
10.1016/S0304-3940(99)00719-3
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Dorsal root ganglion (DRG) neurons that express the neurokinin 1 (NK1) receptor were further characterized immunocytochemically using double-labeling techniques. DRG neurons were isolated from adult rats and cultured for 1-4 days. NK1 receptors on the surface of the neurons were labeled with substance P-gold. Non-peptidergic neurons were labeled with the surface marker isolectin B4 from Griffonia simplicifolia (IB4). Substance P-immunoreactivity was determined using an antibody to substance P. NK1 receptors were not identified on non-peptidergic neurons. The vast majority of the neurons with NK1 receptors showed substance P-immunoreactivity suggesting that NK1 receptors are autoreceptors in many dorsal root ganglion neurons. (C) 1999 Published by Elsevier Science Ireland Ltd. Ail rights reserved.
引用
收藏
页码：175 / 178
页数：4
相关论文
共 50 条
  • [1] Oxaliplatin Depolarizes Isolectin B4-Negative Dorsal Root Ganglion Neurons to Initiate Neuropathic Pain in Mouse
    Wu, B.
    Su, X.
    Tan, Z.
    JOURNAL OF PAIN, 2019, 20 (04): : S27 - S27
  • [2] Activation of the neurokinin-1 receptor by substance P triggers the release of substance P from cultured adult rat dorsal root ganglion neurons
    Tang, He-Bin
    Li, Yu-Sang
    Arihiro, Koji
    Nakata, Yoshihiro
    MOLECULAR PAIN, 2007, 3
  • [3] TIME OF ORIGIN OF SUBSTANCE P-POSITIVE NEURONS IN CHICK DORSAL-ROOT GANGLIA
    SCOTT, SA
    NEUROSCIENCE LETTERS, 1990, 114 (02) : 135 - 140
  • [4] Neurokinin-1 receptor expression in dorsal root ganglion neurons of young rats
    Szucs, P
    Polgar, E
    Spigelman, I
    Porszasz, R
    Nagy, I
    JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM, 1999, 4 (3-4) : 270 - 278
  • [5] Impaired axonal regeneration by isolectin B4-binding dorsal root ganglion neurons in vitro
    Leclere, Pascal G.
    Norman, Emma
    Groutsi, Filitsa
    Coffin, Robert
    Mayer, Ulrike
    Pizzey, John
    Tonge, David
    JOURNAL OF NEUROSCIENCE, 2007, 27 (05): : 1190 - 1199
  • [6] Functional endothelin receptors are selectively expressed in isolectin B4-negative sensory neurons and are upregulated in isolectin B4-positive neurons by neurturin and glia-derived neurotropic factor
    Vellani, Vittorio
    Prandini, Massimiliano
    Giacomoni, Chiara
    Pavesi, Giorgia
    Ravegnani, Laura
    Magherini, Pier Cosimo
    BRAIN RESEARCH, 2011, 1381 : 31 - 37
  • [7] Evidence for the existence of substance P autoreceptor in the membrane of rat dorsal root ganglion neurons
    Hu, HZ
    Li, ZW
    Si, JQ
    NEUROSCIENCE, 1997, 77 (02) : 535 - 541
  • [8] Phosphorylation of TRPV1 by neurokinin-1 receptor agonist exaggerates the capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons
    Tang, He-Bin
    Li, Yu-Sang
    Miyano, Kanako
    Nakata, Yoshihiro
    NEUROPHARMACOLOGY, 2008, 55 (08) : 1405 - 1411
  • [9] High voltage-activated Ca2+ channel currents in isolectin B4-positive and -negative small dorsal root ganglion neurons of rats
    Wu, ZZ
    Pan, HL
    NEUROSCIENCE LETTERS, 2004, 368 (01) : 96 - 101
  • [10] Destruction of dorsal root ganglion neurons by isolectin B4-conjugated saporin (IB4-sap)
    Fukuda, Tomoki
    Ichikawa, Hiroyuki
    Terayama, Ryuji
    Sugimoto, Tomosada
    NEUROSCIENCE RESEARCH, 2006, 55 : S191 - S191
12345