Micro-Raman analysis and AFM imaging of Acidithiobacillus ferrooxidans biofilm grown on uranium ore

被引:20
|
作者
Pradhan, Nilotpala [1 ]
Pradhan, Siddhartha K. [1 ]
Nayak, Bijan B. [1 ]
Mukherjee, Partha S. [1 ]
Sukla, Lala B. [1 ]
Mishra, Barada K. [1 ]
机构
[1] Inst Minerals & Mat Technol, Bhubaneswar 751013, Orissa, India
关键词
Biofilm; Extracellular polymeric substances; Acidithiobacillus ferrooxidans; Raman spectroscopy; Atomic force microscopy;
D O I
10.1016/j.resmic.2008.06.006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Acidithiobacillus ferrooxidans biofilm grown on uranium ore substrate was analyzed by a micro-Raman spectrometer and an atomic force microscope (AFM). The bacterium employed for this study, A. ferrooxidans BMI, was isolated from a uranium mine (Jaduguda, India). Micro-Raman analysis revealed the different constituents of molecular fragments present in microbial cells and in secreted extracellular polymeric substances (EPSs). AFM images clearly revealed bacterial cells surrounded by EPS. From Raman spectral data, the composition of EPS from A. ferrooxidans BMI appeared to be similar to that of EPS secreted in a different Pseudomonas bacterium. (C) 2008 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:557 / 561
页数:5
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