Comparative analysis of eukaryotic cell-free expression systems

被引：7
|
作者：
Hartsough, Emily M. [1 ,2 ]
Shah, Pankti [3 ]
Larsen, Andrew C. [1 ]
Chaput, John C. [1 ,3 ]
机构：
[1] Arizona State Univ, Ctr Personalized Med, Tempe, AZ 85287 USA
[2] Arizona State Univ, Sch Life Sci, Tempe, AZ USA
[3] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ USA
来源：
BIOTECHNIQUES | 2015年 / 59卷 / 03期
关键词：
recombinant protein synthesis; cell-free translation; translation enhancing elements; CAP-INDEPENDENT TRANSLATION; POLY(A) TAIL; IN-VITRO; SEQUENCE; PEPTIDES; PROTEINS;
D O I：
10.2144/000114327
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cell-free protein synthesis (CFPS) allows researchers to rapidly generate functional proteins independent of cell culture. Although advances in eukaryotic lysates have increased the amount of protein that can be produced, the nuances of different translation systems lead to variability in protein production. To help overcome this problem, we have compared the relative yield and template requirements for three commonly used commercial cell-free translation systems: wheat germ extract (WGE), rabbit reticulocyte lysate (RRL), and HeLa cell lysate (HCL). Our results provide a general guide for researchers interested in using cell-free translation to generate recombinant protein for biomedical applications.
引用
收藏
页码：149 / 151
页数：3
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