The genotypes of citrus tristeza virus isolates from China revealed by sequence analysis of multiple molecular markers

被引:10
|
作者
Wu, Guan-wei [1 ,2 ]
Pan, Song [2 ]
Wang, Guo-ping [1 ,2 ]
Tang, Min [2 ]
Liu, Yong [2 ]
Yang, Fan [2 ]
Hong, Ni [1 ,2 ]
机构
[1] Huazhong Agr Univ, Natl Key Lab Agromicrobiol, Wuhan 430070, Hubei, Peoples R China
[2] Huazhong Agr Univ, Coll Plant Sci & Technol, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei, Peoples R China
基金
中国国家自然科学基金;
关键词
MIXED INFECTIONS; COAT PROTEIN; RECOMBINATION; RESTRICTION; DIVERSITY; VIRION;
D O I
10.1007/s00705-012-1475-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genotypes of ten citrus tristeza virus (CTV) isolates from central China were determined by examining multiple molecular markers (MMMs) using 11 primer pairs. The results revealed that one isolate contained a single T30 genotype, two isolates contained a single VT genotype, and the other seven isolates were mixtures of two or more genotypes. Sequence analysis of amplified MMMs showed a high genetic diversity in Chinese CTV populations. The genotypes resembling T36, RB and B165 were identified from Chinese CTV isolates for the first time. Our results suggest that genotype assignment of CTV cannot be based solely on the amplification profiles of MMMs, and sequencing of MMMs is required.
引用
收藏
页码:231 / 235
页数:5
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