Sequence and comparative analysis of the MIP gene in Chinese straw mushroom, Volvariella volvacea

被引:1
|
作者
Chen, Bing-Zhi [1 ]
Gui, Fu [1 ]
Xie, Bao-Gui [1 ]
Zou, Feng [1 ]
Jiang, Yu-Ji [1 ]
Deng, You-Jin [1 ]
机构
[1] Fujian Agr & Forestry Univ, Mycol Res Ctr, Fuzhou, Peoples R China
关键词
Volvariella volvacea; mitochondrial intermediate peptidase; mating-type A gene (mtA); genomic analysis; MATING-TYPE LOCUS; EVOLUTION;
D O I
10.1139/G2012-043
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The mitochondrial intermediate peptidase (MIP) gene is conserved in fungi. It is linked closely with the matingtype A (mtA) gene. In this study, a fragment of the MIP gene in Volvariella volvacea (Bull. ex Fr.) Singer was first cloned by homologue-based cloning technology. Subsequently, the entire MIP DNA sequence (PYd21-MIP) was obtained after the fragment was compared with the genomic data through BLAST analysis. The PYd21-MIP sequence appeared to be homologous with the MIP gene in other fungi. Phylogenetic analysis of PYd21-MIP and other MIP sequences from diverse fungi agreed with the current organism phylogeny. Analysis of protein domains by InterProScan software and motif searching demonstrated that PYd21-MIP encodes a homologous MIP protein. These data support the hypothesis that the PYd21-MIP protein is a Hog-MIP protein homologue from V. volvacea.
引用
收藏
页码:667 / 672
页数:6
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