Genome-wide analysis of differentially expressed mRNAs, lncRNAs, and circRNAs in chicken bursae of Fabricius during infection with very virulent infectious bursal disease virus

被引：15

作者：

Huang, Xuewei ^{[1
]}

Zhang, Junyan ^{[1
]}

Liu, Zengsu ^{[1
]}

Wang, Meng ^{[1
]}

Fan, Xiaolong ^{[1
]}

Wang, Li ^{[1
,2
]}

Zhou, Han ^{[1
,2
]}

Jiang, Yanping ^{[1
,2
]}

Cui, Wen ^{[1
,2
]}

Qiao, Xinyuan ^{[1
,2
]}

Xu, Yigang ^{[1
,2
]}

Li, Yijing ^{[1
,2
]}

Tang, Lijie ^{[1
,2
]}

机构：

[1] Northeast Agr Univ, Coll Vet Med, Changjiang Rd 600, Harbin, Peoples R China

[2] Heilongjiang Key Lab Anim Dis Control & Pharmaceu, Harbin, Peoples R China

来源：

BMC GENOMICS | 2020年 / 21卷 / 01期

关键词：

Infectious bursal disease virus; Bursa of Fabricius; mRNAs; lncRNAs; circRNAs; LONG NONCODING RNAS; CIRCULAR RNAS; ANTIVIRAL RESPONSES; TARGETED DISRUPTION; INNATE IMMUNITY; GENE; IBDV; IDENTIFICATION; ROLES; TRANSCRIPTS;

D O I：

10.1186/s12864-020-07129-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

BackgroundInfectious bursal disease virus (IBDV) causes acute, highly contagious, immunosuppressive, and lethal infectious disease in young chickens and mainly infects the bursa of Fabricius (BF). To investigate interactions between IBDV and its host, RNA sequencing was applied to analyze the responses of the differentially expressed transcriptional profiles of BF infected by very virulent IBDV (vvIBDV).ResultsIn total, 317 upregulated and 94 downregulated mRNAs were found to be significantly differentially expressed in infected chickens, compared to controls. Long non-coding RNA (lncRNA) and circular RNA (circRNA) alterations were identified in IBDV-infected chickens, and significantly different expression was observed in 272 lncRNAs and 143 circRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to assess the functions of significantly dysregulated genes, which showed that the JAK-STAT signaling pathway, the NOD-like receptor signaling pathway, and apoptosis may be activated by IBDV infection. We predicted interactions between differentially expressed genes and produced lncRNA-mRNA and circRNA-miRNA-mRNA regulator network.ConclusionsThe present study identified the expression profiles of mRNAs, lncRNAs, and circRNAs during vvIBDV infection and provides new insights into the pathogenesis of IBDV and antiviral immunity of the host.

引用

页数：15

共 32 条

[31] Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis
M. R. Islam
S. Rahman
M. Noor
E. H. Chowdhury
H. Müller
Archives of Virology, 2012, 157 : 333 - 336
[32] Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis
Islam, M. R.
Rahman, S.
Noor, M.
Chowdhury, E. H.
Mueller, H.
ARCHIVES OF VIROLOGY, 2012, 157 (02) : 333 - 336

← 1 2 3 4 →